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Localization of the gene for P700 chlorophyll a protein in pea chloroplast DNA

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Summary

The gene for the P700 chlorophyll a protein (CPI) has been located in pea chloroplast DNA, using a cell-free coupled transcription-translation system from E. coli programmed with total pea chloroplast DNA and cloned restriction fragments of pea chloroplast DNA. Poly-peptides related to CPI were identified by immunoprecipitation with specific antibodies raised against CPI from pea, and protein A-Sepharose. The gene is located between the genes for the α subunit of ATP synthase and cytochrome f.

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Authors and Affiliations

  1. Botany School, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    Alison G. Smith & John C. Gray

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  1. Alison G. Smith
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  2. John C. Gray
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Communicated by P.T. Emmerson

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Smith, A.G., Gray, J.C. Localization of the gene for P700 chlorophyll a protein in pea chloroplast DNA. Molec. Gen. Genet. 194, 471–476 (1984). https://doi.org/10.1007/BF00425560

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  • DOI: https://doi.org/10.1007/BF00425560

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