Molecular and General Genetics MGG

, Volume 194, Issue 3, pp 416–422 | Cite as

Location and nucleotide sequence of the gene for cytochrome f in wheat chloroplast DNA

  • David L. Willey
  • Christopher J. Howe
  • Anthony D. Auffret
  • Catherine M. Bowman
  • Tristan A. Dyer
  • John C. Gray
Article

Summary

The gene for cytochrome f has been located in wheat chloroplast DNA by hybridisation with a 3.3 kbp BglII fragment of pea chloroplast DNA containing the gene for cytochrome f, by in vitro transcription-translation of cloned restriction fragments of wheat chloroplast DNA and by nucleotide sequence analysis. The gene is located 3 kbp from the 3′ end of the gene for the large subunit of ribulose bisphosphate carboxylase and is transcribed from the same DNA strand. Nucleotide sequence analysis reveals an open reading frame of 320 amino acids, of which 285 amino acid residues comprise the mature polypeptide and 35 amino acid residues probably represent an N-terminal signal sequence. The nucleotide sequence of the wheat gene shows 85% homology with the gene for pea cytochrome f.

Keywords

Nucleotide Sequence Analysis Nucleotide Sequence Polypeptide Amino Acid Residue 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • David L. Willey
    • 1
  • Christopher J. Howe
    • 1
  • Anthony D. Auffret
    • 2
  • Catherine M. Bowman
    • 3
  • Tristan A. Dyer
    • 3
  • John C. Gray
    • 1
  1. 1.Department of BotanyUniversity of CambridgeCambridgeUK
  2. 2.Protein Sequencing Unit, Department of BiochemistryUniversity of LeedsLeedsUK
  3. 3.Plant Breeding InstituteCambridgeUK

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