Summary
Bacteriophage P2 normally requires the products of its early genes A and B for lytic growth in its host, Escherichia coli C. A host mutation, sub-1, which allows P2 to grow without a functional B gene product is described. The sub-1 mutation is recessive and maps at approximately 10 min on the E. coli genetic map.
This is a preview of subscription content, log in via an institution to check access.
References
Bachmann BJ (1983) Linkage map of Escherichia coli K-12. Edition 7 Microbiol Rev 47:180–230
Bertani G, Ljungqvist E (1984) Bacteriophage P2 in genetic maps, vol 3, O'Brien SJ (ed). Cold Spring Harbor Laboratory
Bertani G, Six E (1958) Inheritance of prophage P2 in bacterial crosses. Virology 6:357–381
Blinkowa A, Gawecka G (1979) Mechanism of conjugation II. Characterization of an Hfr dna ts mutant of Escherichia coli. Mol Gen Genet 172:107–111
Calendar R, Lindqvist B, Sironi G, Clark AJ (1970) Characterization of REP- mutants and their interaction with P2 phage. Virology 40:72–83
Carl P (1970) Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet 109:107–122
Chattoraj DK (1978) Sraand-specific break near the origin of bacteriophage P2 DNA replication. Proc Natl Acad Sci USA 75:1685–1689
Christie G, Calendar R (1983) Bacteriophage P2 late promoters. Transcription initiation sites for two late mRNAs. J Mol Biol 167:773–790
Filip CC, Allen JS, Gustafson RA, Allen RG, Walker JR (1974) Bacterial cell division regulation: Characterization of the dnaH locus of Escherichia coli. J Bacteriol 119:443–449
Funnel B, Inman R (1983) Bacteriophage P2 DNA replication. Characterization of the requirement of the gene B product in vivo. J Mol Biol 167:311–334
Friedman DI, Baumann M, Baron LS (1976) Cooperative effects of bacterial mutations affecting λN gene expression. I. Isolation and characterization of a nusB mutant. Virology 73:119–127
Friedman DI, Olson ER, Georgopolous C, Tilly K, Herskowitz I, Banuett F (1984) Interactions of bacteriophage and host macromolecules in the growth of bacteriophage λ. Microbiol Rev 48:299–325
Geisselsoder J (1976) Strand-specific discontinuity in replicating P2 DNA. J Mol Biol 100:13–22
Ghisotti D, Zangrossi S, Sironi G (1983) An Escherichia coli gene required for bacteriophage P2-λ interference. J Virol 48:616–626
Henson JM, Chu H, Irwin CA, Walker JR (1979) Isolation and characterization of dnaX and dnaY temperature-sensitive mutants of Escherichia coli. Genetics 92:1041–1059
Kornberg A (1980) DNA Replication. WH Freeman and Co, San Francisco, pp 383–390
Lengyel J, Calendar R (1974) Control of bacteriophage P2 protein and DNA synthesis. Virology 57:305–313
Levine RS, Taylor MW (1982) Mechanism of adenine toxicity in Escherichia coli. J Bacteriol 149:923–930
Lindahl G (1970) Bacteriophage P2: Replication of the chromosome requires a protein which acts only on the genome that coded for it. Virology 42:522–533
Lindahl G (1974) Characterization of conditional lethal mutants of bacteriophage P2. Mol Gen Genet 128:249–260
Lindqvist BH (1971) Vegetative DNA of temperate phage P2. Mol Gen Genet 110:178–196
Lindqvist BH, Bovre K (1972) Asymmetric transcription of the coliphage P2 genome during infection. Virology 49:690–699
Ricard M, Hirota Y (1969) Effect des sels sur le processus de division cellulaire d'E. coli. CR Acad Sci (Paris) 268:1335–1338
Six EW (1975) The helper dependence of satellite bacteriophage P4: Which gene functions of bacteriophage P2 are needed by P4? Virology 67:249–263
Sunshine M, Thorn M, Gibbs W, Calendar R, Kelly B (1971) P2 phage amber mutants: Characterization by use of a polarity suppressor. Virology 46:691–702
Sunshine M, Usher D, Calendar R (1975) Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli. J Virol 16:284–289
Vales LD, Rabin BA, Chase JW (1983) Isolation and preliminary characterization of Escherichia coli mutants defective in exonuclease VII. J Bacteriol 155:1116–1122
Wiman M, Bertani G, Kelly B, Sasaki I (1970) Genetic map of Escherichia coli strain C. Mol Gen Genet 107:1–31
Wu TT (1966) A model for three point analysis of random general transduction. Genetics 54:405–410
Author information
Authors and Affiliations
Additional information
Communicated by G. Smith
Rights and permissions
About this article
Cite this article
Sunshine, M., Six, E. sub, a host mutation that specifically allows growth of replication-deficient gene B mutants of coliphage P2. Molec. Gen. Genet. 204, 359–361 (1986). https://doi.org/10.1007/BF00425523
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425523