Molecular and General Genetics MGG

, Volume 204, Issue 2, pp 334–340 | Cite as

Heat-shock locus 93D of Drosophila melanogaster: An RNA with limited coding capacity accumulates precursor transcripts after heat shock

  • Bernd Hovemann
  • Uwe Walldorf
  • Rolf-Peter Ryseck


Heat-shock locus 93D of Drosophila melanogaster consists of an internally repetitive and a neighbouring unique region. The unique part contains a promoter that is already active in non-heat-shocked cells but shows fivefold enhanced transcription after stress induction. In third instar larvae, a series of 93D-derived RNA products appear, which might be the result of incomplete processing events. The major RNA species (1.2 kb) is the splicing product of a poly(A)+-containing primary transcript of 1.9 kb. Furthermore, a transcript of high molecular weight is observed, which in addition contains the 3′-flanking 93D-specific ‘TaqI repeat’ sequences. This readthrough transcript is found in the poly(A)+ as well as in the poly(A)- RNA fraction. After severe heat shock, the already limited processing efficiency of 93D RNA is further inhibited. Production of the readthrough transcript, on the other hand, is reduced. DNA sequence analyses of genomic and cDNA sequences reveal that this 93D heat-shock gene contains only a very limited protein-coding capacity. A coding function for the mature 93D heat-shock RNA is therefore questionable.

Key words

D. melanogaster Heat shock RNA splicing 


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  1. Berk AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732Google Scholar
  2. Bonner JJ, Kerby RL (1982) RNA polymerase II transcribes all of the heat shock induced genes of Drosophila melanogaster. Chromosoma 85:93–108Google Scholar
  3. Dangli A, Grond C, Kloetzel P, Bautz EKF (1983) Heat-shock puff 93D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function. EMBO J 2:1747–1751Google Scholar
  4. Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646Google Scholar
  5. Grabowski PJ, Padgett RA, Sharp PA (1984) Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate. Cell 37:415–427Google Scholar
  6. Kozak M (1978) How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell 15:1109–1123Google Scholar
  7. Kozak M (1984) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res 12:857–872Google Scholar
  8. Krainer AR, Maniatis T, Ruskin B, Green MR (1984) Normal and mutant human β-globin pre-mRNA are faithfully and efficiently spliced in vitro. Cell 36:993–1005Google Scholar
  9. Lakhotia SC, Mukherjee T (1982) Absence of novel translation products in relation to induced activity of the 93D puff in Drosophila melanogaster. Chromosomes 85:369–374Google Scholar
  10. Lakhotia SC, Singh AK (1982) Conservation of the 93D puff of Drosophila melanogaster in different species of Drosophila. Chromosoma 86:265–278Google Scholar
  11. Lengyel JA, Ransom LJ, Graham ML, Pardue ML (1980) Transcription and metabolism of RNA from the Drosophila melanogaster heat-shock puff site 93D. Chromosoma 80:237–252Google Scholar
  12. Maniatis T, Fritsch EF, Sambrock J (1982) Molecular cloning, laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  13. Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560Google Scholar
  14. Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res 12:7035–7056Google Scholar
  15. Mohler J, Pardue ML (1984) Mutational analysis of the region surrounding the 93D heat-shock locus of Drosophila melanogaster. Genetics 106:249–265Google Scholar
  16. Moran LA, Chauvin M, Kennedy ME, Korri M, Lowe DG, Nicholson RC, Perry MD (1983) The major heat-shock protein (hsp 70) gene family: related sequences in mouse, Drosophila and yeast. Can J Biochem Cell Biol 61:488–499Google Scholar
  17. Nover L, Hellmund D, Neumann D, Scharf KD, Serfling E (1984) The heat-shock response of eucaryotic cells. Biol Zentralbl 103:357–435Google Scholar
  18. Parker CS, Topol J (1984) A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene. Cell 37:273–283Google Scholar
  19. Pelham HRB (1982) A regulatory upstream promoter element in the Drosophila hsp 70 heat shock gene. Cell 30:517–528Google Scholar
  20. Peters FPAMN, Lubsen NH, Walldorf U, Moormann RJM, Hovemann B (1984) The unusual structure of heat-shock locus 2-48B in Drosophila hydei. Mol Gen Genet 197:392–398Google Scholar
  21. Ryseck RP, Walldorf U, Hovemann B (1985) Two major RNA products are transcribed from heat-shock locus 93D of Drosophila melanogaster. Chromosoma 93:17–20Google Scholar
  22. Walldorf U (1985) Untersuchung entwicklungsspezifisch regulierter und hitzeinduzierter Gene in Drosophila. Ph. D. Thesis, HeidelbergGoogle Scholar
  23. Walldorf U, Richter S, Ryseck RP, Steller H, Edström JE, Bautz EKF, Hovemann B (1984) Cloning of heat-shock locus 93D from Drosophila melanogaster. EMBO J 3:2499–2504Google Scholar
  24. Wu C (1985) An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts. Nature 317:84–87Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Bernd Hovemann
    • 1
  • Uwe Walldorf
    • 1
  • Rolf-Peter Ryseck
    • 1
  1. 1.Zentrum für Molekulare Biologie (ZMBH)HeidelbergGermany

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