Molecular and General Genetics MGG

, Volume 204, Issue 2, pp 237–242 | Cite as

Isolation, characterization and physiological properties of an autolytic-deficient mutant of Streptococcus pneumoniae

  • Rubens López
  • Jose M. Sánchez-Puelles
  • Ernesto García
  • Jose Luís García
  • Concepción Ronda
  • Pedro García
Article

Summary

A spontaneous mutation in the gene lyt encoding the pneumococcal autolysin has been characterized. This mutation, named lyt-32, which behaves as a high-efficiency marker in pneumococcal transformation, is a single base pair GC deletion causing the appearance of two consecutive termination codons in the amino terminal part of the sequence of the autolysin gene. The mutant lyt gene did not code for a polypeptide of relative molecular mass corresponding to the pneumococcal E form amidase in Escherichia coli maxicells. Pneumococcal cells containing the lyt-32 mutation (M32) were fully transformable, multiplied at a normal growth rate forming small chains and showed a tolerant response when treated with beta-lactam antibiotics. Strain M32 represents the first example of a mutant of Streptococcus pneumoniae completely lacking amidase as a consequence of an alteration in the structural gene coding for the pneumococcal autolysin.

Key words

Autolysins Pneumococcal amidase Frame shift mutation Recombinant plasmids maxicells 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Rubens López
    • 1
  • Jose M. Sánchez-Puelles
    • 1
  • Ernesto García
    • 1
  • Jose Luís García
    • 1
  • Concepción Ronda
    • 1
  • Pedro García
    • 1
  1. 1.Centro de Investigaciones Biológicas C.S.I.C.MadridSpain

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