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Molecular and General Genetics MGG

, Volume 204, Issue 2, pp 229–236 | Cite as

Separation and analysis of the RNA polymerase binding sites of a complex Bacillus subtilis promoter

  • Stuart F. J. Le Grice
  • Chu-Chih Shih
  • Frederick Whipple
  • Abraham L. Sonenshein
Article

Summary

The Bacillus subtilis veg promotor site was shown to be composed of two neighboring RNA polymerase binding sites, only one of which (“Site I”) produces a transcript. The relationship between these sites has been investigated following (a) insertion of ten base pairs of DNA between the sites and subsequent cloning of each site independent of the other, and (b) deletion of DNA sequences upstream from the productive site. The effect of DNA insertion was to permit transcription initiation from the previously nonproductive Site II, in either the presence or absence of Site I. This could be attributed to insertion of a purine residue seven base pairs dowstream from the Pribnow box of Site II. Transcription from Site I was slightly increased in the presence of Site II cis, but was still efficient in the absence of Site II, indicating that there is no absolute dependence on Site II for in vitro transcription from Site I.

Key words

Bacillus Promoters Transcription RNA polymerase 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Stuart F. J. Le Grice
    • 1
  • Chu-Chih Shih
    • 1
  • Frederick Whipple
    • 1
  • Abraham L. Sonenshein
    • 1
  1. 1.Department of Molecular Biology and MicrobiologyTufts University Health Sciences CampusBostonUSA

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