Archives of Microbiology

, Volume 152, Issue 6, pp 606–610 | Cite as

Carbon catabolism in continuous cultures and bacteroids of Rhizobium leguminosarum MNF 3841

  • I. A. McKay
  • M. J. Dilworth
  • A. R. Glenn
Original Papers


Bacteroids of R. leguminosarum MNF3841 isolated from pea nodules using Percoll gradients had activities of TCA cycle enzymes up to 6-fold higher than those measured in free-living cells grown on fumarate or sucrose. Activities of sugar catabolic enzymes on the other hand were 2–14-fold lower in isolated bacteroids than in sucrose-grown free-living cells. In continuous culture, cells of strain MNF3841 grown on sucrose under Pi limitation had 2–3-fold higher activities of invertase, glucose-6-phosphate dehydrogenase, the Entner-Doudoroff enzymes and 6-phosphogluconate dehydrogenase, than cells grown on fumarate. With one exception O2 limited cultures had similar activities of the carbon catabolic enzymes to Pi-limited cultures grown in the same substrate. Glucose-6-phosphate dehydrogenase in O2-limited cells grown of fumarate was 50% lower than in Pi-limited cells. Co-utilization of fumarate and sucrose occurred with chemostat cultures supplied with both under a variety of conditions.

Key words

Rhizobium leguminosarum Catabolic enzymes Bacteroids Percoll gradients Chemostats Substrate consumption 







phosphoenolpyruvate carboxy kinase


N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulphonic acid]


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • I. A. McKay
    • 1
  • M. J. Dilworth
    • 1
  • A. R. Glenn
    • 1
  1. 1.School of Biological and Environmental SciencesMurdoch UniversityMurdoch

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