Summary
A mutant strain of E. coli displaying altered regulation of the recA gene was isolated as a revertant of a lexA3 recA200 double mutant which showed improved DNA repair and recombination functions. The mutation (alc-24) was located between srl and recA200 and caused synthesis of high levels of recA protein in both lexA + and lexA3 strains. The possibility that alc-24 defines the regulatory site for recA is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36:525–557
Bachmann BK, Low B, Taylor AL (1976) Recalibrated linkage of Escherichia coli K-12. Bacteriol Rev 40:116–167
Emmerson PT, West SC (1977) Identification of protein X of Escherichia coli as the recA +/tif + gene product. Mol Gen Genet 155:77–85
Gudas LJ, Mount DW (1977) Identification of the recA (tif) gene product of Escherichia coli. Proc Natl Acad Sci USA 74:5280–5284
Gudas LJ, Pardee AB (1976) DNA synthesis inhibition and the induction of protein X in Escherichia coli. J Mol Biol 101:459–477
Horii T, Ogawa T, Ogawa H (1980). Organisation of the recA gene of Escherichia coli. Proc Natl Acad Sci USA 77:313–317
Little JW (1979) Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein. Mol Gen Genet 177:13–22
Lloyd RG (1974) The segregation of the SbcA and Rac phenotypes in an Escherichia coli recB − mutant. Mol Gen Genet 134:249–259
Lloyd RG, Barbour SD (1974) The genetic location of the sbcA gene of Escherichia coli. Mol Gen Genet 134:157–171
Lloyd RG, Low B, Godson GN, Birge EA (1974) Isolation and characterisation of an Escherichia coli K-12 mutant with a temperature-sensitive RecA− phenotype. J Bacteriol 120:407–415
McEntec K (1977) Protein X is the product of the recA gene of Escherichia coli. Proc Natl Acad Sci USA 74:5275–5279
Mount DW (1977) A mutant of Escherichia coli showing constitutive expression of the lysogenic induction and error-prone DNA repair pathways. Proc Natl Acad Sci USA 74:300–304
Mount DW, Little JW, Edmiston SH (1980) Influence of plasmids carring the lexA gene on DNA repair and related processcs in Escherichia coli K-12. Mol Gen Genet 177:477–483
Mount DW, Low KB, Edmiston SJ (1972) Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. J Bacteriol 112:886–893
Sancar A, Rupp WD (1979) Physical map of the recA. gene. Proc Natl Acad Sci USA 76:3144–3148
West SC, Emmerson PT (1977) Induction of protein synthesis in Escherichia coli following UV or γ-irradiation, Mitomycin C treatment of tif expression. Mol Gen Genet 151:57–67
Witkin EM (1976) Ultraviolet mutagenesis and inducible deoxyribonucleic acid repair in Escherichia coli. Bacteriol Rev 40:869–907
Author information
Authors and Affiliations
Additional information
Communicated by B.A. Bridges
Rights and permissions
About this article
Cite this article
Thomas, A., Lloyd, R.G. Altered regulation of the recA gene in Escherichia coli strains carrying a recA-linked suppressor of lexA . Molec. Gen. Genet. 179, 355–358 (1980). https://doi.org/10.1007/BF00425464
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425464