Molecular and General Genetics MGG

, Volume 200, Issue 2, pp 240–246 | Cite as

A molecular genetic analysis of insertions in the bronze locus in maize

  • Hugo K. Dooner
  • Edward Weck
  • Sharon Adams
  • Edward Ralston
  • Mitchell Favreau
  • James English


We have undertaken a combined molecular and genetic analysis of the bronze (bz) locus in maize. The bz locus was isolated by a two-step approach involving sequences from the transposable elements Dissociation (Ds) and Activator (Ac). A restriction map of the bz region is presented, identifying the transcribed region and the direction of transcription. We have mapped the sites of two Ds insertions, bz-m2 (DI) and bz-m1, physically and genetically. bz-m2 (DI) is a 3.5 kb insertion in the transcribed region that appears to have arisen by an internal deletion in the 4.5 kb Ac insertion in the mutant bz-m2. bz-m1 is a 1.1 kb insert in the 5′ end of the gene. By correlating the genetic and physical maps, we have been able to orient the restriction map relative to the centromere in chromosome 9 and have obtained a rough estimate of the physical length of a unit of genetic recombination in a microregion of the maize genome.


Maize Recombination Genetic Analysis Transposable Element Molecular Genetic Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Aviv H, Leder P (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci US 69: 1408–1412Google Scholar
  2. Beckett JB, Burnham CR, Coe EH, Maguire MP, Patterson EP, Phillips RL (1978) Cytogenetic working map. Maize Genet Coop Newsl 52: 129–145Google Scholar
  3. Behrens U, Fedoroff N, Laird A, Muller-Neumann M, Starlinger P, Yoder J (1984) Cloning of the Zea mays controlling element Ac from the wx-m7 allele. Mol Gen Genet 194: 346–347Google Scholar
  4. Benton W, Davis R (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196: 180–182Google Scholar
  5. Coe EH, Hoisington D, Neuffer G (1984) Linkage map of corn Zea mays L). In: O'Brien SJ (ed) Genetic Maps 1984. Cold Spring Harbor Laboratory, New York pp 491–507Google Scholar
  6. Coe EH, Neuffer MG (1977) The genetics of corn. In: Sprague GF (ed) Corn and corn improvement. American Soc of Agronomy, Madison, pp 111–223Google Scholar
  7. Dooner HK (1981) Regulation of the enzyme UFGT by the controlling element Ds in bz-m4, an unstable mutant in maize. Cold Spring Harbor Symp Quant Biol 45: 457–462Google Scholar
  8. Dooner HK, Nelson OE (1977a) Genetic control of UDP glucoseflavonol 3-O-glucosyltransferase in the endosperm of maize. Biochem Genet 15: 509–515Google Scholar
  9. Dooner HK, Nelson OE (1977b) Controlling element-induced alteration in UDPG: flavonoid glucosyltransferase, the enzyme specified by the bronze locus in maize. Proc Natl Acad Sci US 74: 5623–5627Google Scholar
  10. Dooner HK, Nelson OE (1979) Heterogeneous flavonoid glucosyltransferases in purple derivatives from a controlling element-suppressed bronze mutant in maize. Proc Natl Acad Sci US 76: 2369–2371Google Scholar
  11. Döring HP, Tillmann E, Starlinger P (1984) DNA sequence of the maize transposable element Dissociation (Ds). Nature 307: 127–130Google Scholar
  12. Fedoroff N, Wessler S, Shure M (1983) Isolation of the transposable maize controlling elements Ac and Ds. Cell 35: 235–242Google Scholar
  13. Fedoroff N, Furtek D, Nelson O (1984) Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci US 81: 3825–3829Google Scholar
  14. Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842Google Scholar
  15. Galbraith D, Harkins K, Maddox J, Ayres N, Sharma D, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051Google Scholar
  16. Geiser M, Weck E, Doring H-P, Werr W, Courage-Tebbe U, Tillmann E, Starlinger P (1982) Genomic clones of a wild-type allele and a transposable element-induced mutant allele of the sucrose synthase gene of Zea mays L. EMBO 1: 1455–1460Google Scholar
  17. Gerats A, Bussard J, Coe EH, Larson R (1984) Influence of B and Pl on UDPG: flavonoid-3-O-glucosyltransferase in Zea mays L. Biochem Genet 22: 1161–1169Google Scholar
  18. Hake S, Walbot V (1980) The genome of Zea mays, its organization and homology to related grasses. Chromosoma 79: 251–270Google Scholar
  19. Karn J, Brenner S, Barnett L, Cesarini G (1980) Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci US 77: 5172–5176Google Scholar
  20. Larson R, Coe EH (1977) Gene-dependent flavonoid glucosyltransferase in maize. Biochem Genet 15: 153–156Google Scholar
  21. Maniatis T, Fritsch E, Sambrook (1982) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  22. McClintock B (1951) Mutable loci in maize. Carnegie Inst Wash Yrbk 50: 174–181Google Scholar
  23. McClintock B (1952) Chromosome organization and gene expression. Cold Spring Harbor Symp Quant Biol 16: 13–47Google Scholar
  24. McClintock B (1955) Controlled mutation in maize. Carnegie Inst Wash Yrbk 54: 245–255Google Scholar
  25. McClintock B (1956) Mutation in maize. Carnegie Inst Wash Yrbk 55: 323–332Google Scholar
  26. McClintock B (1962) Topographical relations between elements of control systems in maize. Carnegie Inst Wash Yrbk 61: 448–461Google Scholar
  27. McClintock B (1963) Further studies of gene-control systems in maize. Carnegie Inst Wash Yrbk 62: 486–493Google Scholar
  28. Mottinger J (1973) Unstable mutants of bronze induced by premeiotic X-ray treatment in maize. Theor Appl Genet 43: 190–195Google Scholar
  29. Mueller-Neumann M, Yoder JI, Starlinger P (1984) The DNA sequence of the transposable element Ac of Zea mays L. Mol Gen Genet 198: 19–24Google Scholar
  30. Murray M, Thompson W (1980) Rapid isolation of large molecular weight plant DNA. Nucleic Acid Res 8: 4321–4326Google Scholar
  31. Pohlman R, Fedoroff N, Messing J (1984a) The nucleotide sequence of the maize controlling element Activator. Cell 37: 635–643Google Scholar
  32. Pohlman R, Fedoroff N, Messing J (1984b) Correction: nucleotide sequence of Ac. Cell 39: 417Google Scholar
  33. Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517Google Scholar
  34. Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic primers. Gene 19: 259–268Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Hugo K. Dooner
    • 1
  • Edward Weck
    • 1
  • Sharon Adams
    • 1
  • Edward Ralston
    • 1
  • Mitchell Favreau
    • 1
  • James English
    • 1
  1. 1.Advanced Genetic SciencesOaklandUSA

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