Archives of Microbiology

, Volume 138, Issue 1, pp 15–17 | Cite as

Isolation and partial characterization of plasmid DNA from Arthrobacter oxidans

  • Roderich Brandsch
  • Karl Decker
Original Papers

Abstract

A method for the extraction of the high molecular weight plasmid AO 1 from the gram-positive soil bacterium Arthrobacter oxidans is presented.

Following digestion of this DNA with the restriction endonucleases Accl, Bam HI, Eco RI and Hind III, an average molecular mass of 157.8 kb was estimated. This value is in good agreement with the 160 kb size determined previously by electron microscopy (Brandsch et al. 1982).

Using the same method, no plasmid DNA was found in strains of the genus Arthrobacter which do not degrade nicotine, e.g., A. albidus, A. globiformis and A. auricans.

Key words

Arthrobacter Nicotine degradation Plasmid 

Abbreviations

EDTA

ethylenediaminetetraacetic acid

Kb

kilobasepairs

SDS

sodium dodecyl sulfate

Tris

Tris-(hydroxymethyl)-aminomethan

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Roderich Brandsch
    • 1
  • Karl Decker
    • 1
  1. 1.Biochemisches Institut der Albert-Ludwigs-UniversitätFreiburgGermany

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