Abstract
Nitrogen-limited cells of the obligate chemolithotroph Thiobacillus neapolitanus formed an intracellular polymer during growth in the chemostat. This polymer was isolated and characterized as a branched polyglucose composed of units joined by α-1→4 and α-1→6 linkages. Polyglucose in T. neapolitanus can be considered a storage compound since formation of this compound took place during excess of energy and CO2 whilst shortage of CO2 resulted in rapid breakdown of polyglucose. Moreover the breakdown of polyglucose generated metabolically useful energy as could be demonstrated by polyglucose-dependent protein synthesis. Possession of polyglucose did not influence the viability of T. neapolitanus during prolonged periods of energy starvation. Activities of key enzymes of the oxidative pentose phosphate cycle, glucose-6-phosphatedehydrogenase and 6-phospho-gluconate-dehydrogenase, were demonstrated in cell free extracts of T. neapolitanus and appeared to increase 5- and 3-fold, respectively, during growth on NO -3 instead of NH +4 as a nitrogen source.
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References
Barker SA, Bourne EJ, Whiffen DH (1956) Use of infrared analysis in the determination of carbohydrate structure. Meth Biochem Analys 3:213
Beauclerk AAD, Smith AJ (1978) Transport of d-glucose and 3-O-methyl-d-glucose in the cyanobacteria Aphanocapsa 6714 and Nostoc strain mac. Eur J Biochem 82:187–197
Beudeker RF, Cannon GC, Kuenen JG, Shively JM (1980) Relations between d-ribulose-1,5-bisphosphate carboxylase, carboxysomes and CO2-fixing capacity in the obligate chemolithotroph Thiobacillus neapolitanus, grown under different limitations in the chemostat. Arch Microbiol 124:185–189
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt Biochem 72:248–254
Cripps RE (1973) Microbial metabolism of thiophen-2-carboxylate. Biochem J 134:353–366
Dawes EA, Ribbons DW (1963) Endogenousmetabolism and survival of Escherichia coli in aqueous suspension. J Applied Bacteriol 26:VI
Dawes EA, Senior PJ (1973) The role and regulation of energy reserve polymers in micro-organisms. Advanc Microb Physiol 10:136–266
Doolittle WF (1979) The cyanobacterial genome, its expression, and that control of that expression. Advanc Microb Physiol 20:1–102
Fairnbairn NJ (1953) A modified anthrone reagent. Chemistry and Industry 1953:86
Goa J (1953) A microbiuret method for protein determination; determination of total protein in cerebrospinal fluid. Scand J Clin Lab Invest 5:218
Herbert D (1961) The chemical composition of microorganisms as a function of their environment. In: Meynell GG, Gooder H (eds) Symp Soc Gen Microbiol, Vol 11 University Press, Cambridge, England, pp 391–416
Kuenen JG, Veldkamp H (1973) Effects of organic compounds on growth of chemostat cultures of Thiomicrospira pelophila, Thiobacillus thioparus and Thiobacillus neapolitanus. Arch Mikrobiol 94:173–190
Lehmann M, Wöber G (1976) Accumulation, mobilization and turnover of glycogen in the blue-green bacterium Anacystis nidulans. Arch Microbiol 111:93–97
Linton JD, Cripps RE (1978) The occurrence and identification of intracellular polyglucose storage granules in Methylococcus NCIB 11083 grown in chemostat culture on methane. Arch Microbiol 117:41–48
Matin A, Rittenberg SC (1971) Enzymes of carbohydrate metabolism in Thiobacillus species. J Bacteriol 107:179–186
Matin A (1978) Organic nutrition of chemolithotrophic bacteria. Ann Rev Microbiol 32:433–469
Oren A, Shilo M (1979) Anaerobic heterotrophic dark metabolism in the cyanobacterium Oscillatoria limnetica: sulphur respiration and lactate fermentation. Arch Microbiol 122:77–84
Pelroy RA, Rippka R, Stanier RY (1972) Metabolism of glucose by unicellular blue-green algae. Arch Mikrobiol 87:303–323
Postgate JR (1969) Viable counts and viability. In: Norris JR, Ribbons DW (eds) Methods in microbiology, Vol 1. Academic Press. New York London, pp 611–628
Sarkissian GS, Fowler MW (1974) Interrelationship between nitrate assimilation and carbohydrate metabolism in plant roots. Planta (Berl) 119:335–349
Schade HAR (1973) On the staining of glycogen for electron microscopy with polyacids of tungsten and molybdenum. I. Direct staining of sections of osmium fixed and Epon embedded mouse liver with aqueous solutions of phosphotungstic acid (PTA). In: Wisse E, Daems WTh, Molenaar I, Duyn P van (eds) Electron microscopy and cytochemistry. Elseviers North Holland Publishing Company, Amsterdam, pp 263–266
Sietsma JH, Eveleigh DE, Haskins RH (1969) Cell wall composition and protoplast formation of some Oomycetes species. Biochim Biophys Acta 184:306–317
Smith AJ, Hoare DS (1977) Specialist phototrophs, lithotrophs and methylotrophs: a unity among a diversity of prokaryotes. Bacterial Rev 41:419–448
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Van Houte J, Jansen HM (1970) Role of glycogen in survival of Streptococcus mitis. J Bacteriol 101:1083–1085
Vishniac W, Santer M (1957) The thiobacilli. Bacteriol Rev 26:168–195
Wilkinson JF (1959) The problem of energy-storage compounds in bacteria. Exptl Cell Res Suppl 7:111–130
Wood AP, Kelly DP, Thurnston CF (1977) Simultaneous operation of three catabolic pathways in the metabolism of glucose by Thiobacillus A2. Arch Microbiol 113:265–275
Zevenhuizen LPTM (1966) Function, structure and metabolism of the intracellular polysaccharide of Arthrobacter. Doctoral thesis, Wageningen
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Beudeker, R.F., Kerver, J.W.M. & Kuenen, J.G. Occurrence, structure and function of intracellular polyglucose in the obligate chemolithotroph Thiobacillus neapolitanus . Arch. Microbiol. 129, 221–226 (1981). https://doi.org/10.1007/BF00425255
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DOI: https://doi.org/10.1007/BF00425255