Abstract
It was found that hydroxyurea, l-arginine and l-citrulline respectively significantly stimulated the formation of d-cycloserine in Streptomyces garyphalus. The formation of [14C]-hydroxyurea by washed cells was demonstrated after incubation with l-[guanido-14C]-arginine and l-[ureido-14C]-citrulline. The 15N of H2NCO15NHOH was incorporated to 40% in d-cycloserine. The mass spectrum as well as the 15N NMR spectrum of labelled N,2-dicarbobenzyloxy-d-cycloserine derived from [15N]-hydroxyurea showed that hydroxyurea was the source of the heterocyclic nitrogen in the biosynthesis of d-cycloserine.
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Svensson, ML., Valeria, K. & Gatenbeck, S. Hydroxyurea, a natural metabolite and an intermediate in d-cycloserine biosynthesis in streptomyces garyphalus . Arch. Microbiol. 129, 210–212 (1981). https://doi.org/10.1007/BF00425252
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DOI: https://doi.org/10.1007/BF00425252