Summary
The oxygen consumption of an anaerobically grown cell suspension of E. coli which has been aerated in a solution of casein hydrolysate for 2 h exceeds by about 4 to 5 times that of a suspension which has been gassed with nitrogen for the same period. Chloramphenicol, puromycin, rifampicin, hydroxylamine, and glucose block the formation of respiratory activity during aeration; cycloheximide and actinomycin D are inactive in blocking. The repressive effect of glucose can partially be relieved by cyclic adenosine-3′, 5′-monophosphate. An aerated suspension incorporates 4 to 5 times more of the porphyrin precursor 3H-δ-aminolevulinic acid than a suspension gassed with nitrogen. The above mentioned inhibitors, with the exception of cycloheximide and actinomycin D, partially repress this incorporation.
Zusammenfassung
Der Sauerstoff-Verbrauch einer Suspension anaerob gewachsener Zellen von E. coli, die 2 Std in einer Caseinhydrolysatlösung belüftet wurde, ist etwa 4–5mal höher als der einer gleichzeitig mit Stickstoff begasten Suspension. Chloramphenicol, Puromycin, Rifampicin, Hydroxylamin und Glucose verhindern die Ausbildung der Atmungsaktivität während der Belüftung, Cycloheximid und Actinomycin D dagegen nicht. Der Hemmeffekt der Glucose kann durch cyclisches Adenosin-3′,5′-monophosphat teilweise aufgehoben werden. Eine belüftete Suspension baut die Porphyrin-Vorstufe 3H-δ-Aminolävulinsäure etwa 4–5 mal stärker ein als eine mit Stickstoff begaste. Die oben genannten Inhibitoren, mit Ausnahme von Cycloheximid und Actinomycin D, hemmen diesen Einbau in unterschiedlich starkem Maße.
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Hillebrand, E., Kamp, H. & Hollmann, S. Durch Belüftung induzierte respiratorische Aktivität bei Escherichia coli . Archiv. Mikrobiol. 86, 193–202 (1972). https://doi.org/10.1007/BF00425231
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DOI: https://doi.org/10.1007/BF00425231