Abstract
The F420-reducing hydrogenase of Methanococcus voltae, which takes part in the terminal reduction step of methanogenesis, was localized in situ in ultrathin sections. This result was obtained by the immuno-gold technique using a high titer antiserum raised against the purified enzyme. Its specifity for the hydrogenase was shown by Western blot analysis. The hydrogenase of M. voltae was found to be membrane-associated.
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Abbreviations
- ELISA:
-
Enzyme linked immuno sorbent assay
- F420 :
-
8-hydroxy-5-deazaflavin
References
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Muth, E. Localization of the F420-reducing hydrogenase in Methanococcus voltae cells by immuno-gold technique. Arch. Microbiol. 150, 205–207 (1988). https://doi.org/10.1007/BF00425164
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DOI: https://doi.org/10.1007/BF00425164