Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus
- 27 Downloads
The amino acid incorporation and α-aminoisobutyric acid (AIB) uptake of an alkalophilic Bacillus grown at pH 8.2 (the pH 8-bacteria) were much less pH dependent (less alkalophilic) than those of the organisms grown at pH 10.0 (the pH 10-bacteria), respectively. The rate of AIB uptake of the pH 10-bacteria was almost the same as that of the pH 8-bacteria, while the rate of amino acid incorporation of the pH 10-bacteria was higher than that of the pH 8-bacteria in alkaline environments. The colloidal titration with clupein showed that the amount of negative charge on the pH 10-bacteria was greater than that of the pH 8-bacteria in alkaline environments. Considerable difference in protein composition was observed between the membranes of the pH 8-and 10-bacteria, while no difference was observed in phospholipid composition.
Key wordsAlkalophilic Bacillus Alkalophilicity Loss of alkalophilicity Membrane proteins pH of culture medium
Unable to display preview. Download preview PDF.
- Ames, G. F.: Lipids of Salmonella typhimurium and Escherichia coli: Structure and metabolism. J. Bact. 95, 833–843 (1968)Google Scholar
- Bartlett, G. R.: Phosphorus assay in column chromatography. J. biol. Chem. 234, 466–468 (1959)Google Scholar
- Blank, M. L., Schmit, J. A., Privett, O. S.: Quantitative analysis of lipids by thin-layer chromatography. J. Amer. Oil Chem. Soc. 41, 371–376 (1964)Google Scholar
- Dittmer, J. C., Lester, R. L.: A simple specific spary for the detection of phospholipids on thin-layer chromatograms. J. Lipid Res. 5, 126–127 (1964)Google Scholar
- Fairbanks, G., Steck, T. L., Wallach, D. F. H.: Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10, 2606–2616 (1971)Google Scholar
- Haest, C. W. M., Degier, J., Op den Kamp, J. A. F., Bartels, P., van Deenen, L. L. M.: Changes in permeability of Staphylococcus aureus and derived liposomes with varying lipid composition. Biochim. biophys. Acta (Amst.) 255, 720–733 (1972)Google Scholar
- Kates, M., Baxter, R. M.: Lipid composition of mesophilic and psychrophilic yeast (Candida species) as influenced by environmental temperature. Canad. J. biochem. Physiol. 40, 1213–1227 (1962)Google Scholar
- Lowry, O. H., Roscbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)Google Scholar
- Ohta, K., Kiyomiya, A., Koyama, N., Nosoh, Y.: The basis of the alkalophilic property of a species of Bacillus. J. gen. Microbiol. 86, 259–266 (1975)Google Scholar
- Op den Kamp, J. A. F., van Iterson, W., van Deenen, L. L. M.: Phospholipid composition of Bacillus subtitis. J. Bact. 99, 298–303 (1969)Google Scholar
- Terayama, H.: Surface electric charge of ascites hepatomas and the dissociation of islands of tumor cells. Exp. Cell Res. 28, 113–119 (1962)Google Scholar