Archives of Microbiology

, Volume 109, Issue 1–2, pp 105–108 | Cite as

Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus

  • Noriyuki Koyama
  • Yoshiaki Nosoh


The amino acid incorporation and α-aminoisobutyric acid (AIB) uptake of an alkalophilic Bacillus grown at pH 8.2 (the pH 8-bacteria) were much less pH dependent (less alkalophilic) than those of the organisms grown at pH 10.0 (the pH 10-bacteria), respectively. The rate of AIB uptake of the pH 10-bacteria was almost the same as that of the pH 8-bacteria, while the rate of amino acid incorporation of the pH 10-bacteria was higher than that of the pH 8-bacteria in alkaline environments. The colloidal titration with clupein showed that the amount of negative charge on the pH 10-bacteria was greater than that of the pH 8-bacteria in alkaline environments. Considerable difference in protein composition was observed between the membranes of the pH 8-and 10-bacteria, while no difference was observed in phospholipid composition.

Key words

Alkalophilic Bacillus Alkalophilicity Loss of alkalophilicity Membrane proteins pH of culture medium 



Amino-isobutyric acid


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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Noriyuki Koyama
    • 1
  • Yoshiaki Nosoh
    • 1
  1. 1.Laboratory of Chemistry of Natural ProductsTokyo Institute of TechnologyMeguroku, TokyoJapan

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