Abstract
Nucleic acids isolated from dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two “ribosomal-associated” species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two “ribosomal-associated” RNA components. Molecular weights of 1.20, 0.67, 0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.
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References
Bishop, D. H. L., Claybrook, J. R., Spiegelman, S.: Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J. molec. Biol. 26, 373–387 (1967)
Boedtker, H.: Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde. J. molec. Biol. 35, 61–70 (1968)
Brakke, M. K., Van Pelt, N.: Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Analyt. Biochem. 38, 56–64 (1970a)
Brakke, M. K., Van Pelt, N.: Properties of infectious ribonucleic acid from wheat streak mosaic virus. Virology 42, 699–706 (1970b)
Brambl, R. M.: Protein synthesis during fungal spore germination: Evidence that the ungerminated conidiospores of Botryodiplodia theobromae contain a messenger ribonucleic acid Ph.D. thesis, University of Nebraska (1969)
Brambl, R. M., Van Etten, J. L.: Protein synthesis during fungal spore germination. V. Evidence that the ungerminated conidiospores of Botryodiplodia theobromae contain messenger ribonucleic acid. Arch. Biochem. Biophys. 137, 442–452 (1970)
Darnell, J. E., Jelinek, W. R., Molloy, G. R.: Biogenesis of mRNA: Genetic regulation in mammalian cells. Science 181, 1215–1221 (1973)
Edelman, M., Verma, I., Littauer, U.: Mitochondrial ribosomal RNA from Aspergillus nidulans: Characterization of a novel molecular species. J. molec. Biol. 49, 67–83 (1970)
Van Etten, J. L., Dunkle, L. D., Knight, R. H.: Nucleic acids and fungal spore germination. In: The fungal spore, form and function (D. J. Weber, W. H. Hess, eds.), pp. 243–300. New York-London: Wiley 1976
Van Etten, J. L., Vidaver, A. K., Koski, R. K., Semancik, J. S.: RNA polymerase activity associated with bacteriophage. J. Virol. 12, 464–471 (1973)
Gaubatz, J., Cutler, R. G.: Hybridization of ribosomal RNA labeled to high specific radioactivity with dimethyl sulfate. Biochemistry 14, 760–765 (1975)
Greenberg, J. R.: Messenger RNA metabolism of animal cells. J. Cell Biol. 64, 269–288 (1975)
Hesler, T. L., McLaughlin, C. S.: Small ribonucleic acid molecules produced during ribosome biosynthesis in Saccharomyces cerevisiae. J. biol. Chem. 250, 2003–2007 (1975)
Lane, L. C.: The components of barley stripe mosaic and related viruses. Virology 58, 323–333 (1974)
Lovett, J. S., Haselby, J. A.: Molecular weights of the ribosomal ribonucleic acid of fungi. Arch. Mikrobiol. 80, 191–204 (1971)
Nakazato, H., Edmonds, M.: Purification of messenger RNA and heterogeneous nuclear RNA containing poly(A) sequences. In: Methods in enzymology, Vol. 29 (L. Grossman, K. Moldave, eds.), pp. 431–443. New York-London: Academic Press 1974
Pene, J., Knight, E., Darnell, J. E.: Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J. molec. Biol. 33, 609–623 (1968)
Roheim, J. R., Knight, R. H., Van Etten, J. L.: Synthesis of ribonucleic acids during the germination of Rhizopus stolonifer sporangiospores. Develop. Biol. 41, 137–145 (1974)
Shih, D. S., Lane, L. C., Kaesberg, P.: Origin of the small component of brome mosaic virus RNA. J. molec. Biol. 64, 353–362 (1972)
Sy, J., McCarty, K. S.: Characterization of 5.8-S RNA from a complex with 26-S ribosomal RNA from Arbacia punctulata. Biochim. biophys. Acta (Amst.) 199, 86–94 (1970)
Uden, S. A., Kaufman, K., Warner, J. R.: Small ribosomal ribonucleic acid species of Saccharomyces cerevisiae. J. Bact. 105, 101–106 (1971)
Uden, S. A., Warner, J. R.: Ribosomal RNA synthesis in Saccharomyces cerevisiae. J. molec. Biol. 65, 227–242 (1972)
Vogel, H. J.: A convenient growth medium for Neurospora (medium N). Microbial Genet. Bull. 13, 515–522 (1956)
Zaitlin, M., Hariharasubramanian, V.: An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels. Analyt. Biochem. 35, 296–297 (1970)
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Published with the approval of the Director as paper no. 5006, Journal Series, Nebraska Agricultural Experiment Station. The work was conducted under Nebraska Agricultural Experiment Station Project no. 21-17.
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Knight, R.H., van Etten, J.L. Characteristics of ribonucleic acids isolated from Botryodiplodia theobromae pycnidiospores. Arch. Microbiol. 109, 45–50 (1976). https://doi.org/10.1007/BF00425111
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DOI: https://doi.org/10.1007/BF00425111