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Energy metabolism of Bdellovibrio bacteriovorus

II. P/O ratio and ATP pool turnover rate

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Abstract

The P/O ratio of Bdellovibrio bacteriovorus, strain Bd 109 Sa, was evaluated by two different methods based on the determination of energy-rich phosphate bonds and either NADH oxidation or oxygen-uptake. P/O values calculated on the basis of NADH oxidation were up to 6, which has to be regarded as being overestimated. P/O values calculated from energy-rich phosphate bonds and oxygen uptake were around 2. The P/O values determined for Escherichia coli B were similar. The loss of phosphorylation efficiency at one site is discussed.

The ATP pool turnover rate of Bdellovibrio was 8/min during endogenous respiration and 24/min during substrate respiration. The corresponding values in Escherichia coli B were 3/min and 38/min.

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This study was performed at the University of Hamburg (Institut für Allgemeine Botanik, Abteilung Mikrobiologic).

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Gadkari, D., Stolp, H. Energy metabolism of Bdellovibrio bacteriovorus . Arch. Microbiol. 108, 125–132 (1976). https://doi.org/10.1007/BF00425102

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