Archives of Microbiology

, Volume 121, Issue 3, pp 271–275 | Cite as

The amino acid sequence of the peptide moiety of the pseudomurein from Methanobacterium thermoautotrophicum

  • Helmut König
  • Otto Kandler


The amino acid sequence of the peptide subunits of the peptide moiety of the sacculus polymer (pseudomurein) of Methanobacterium thermoautotrophicum was elucidated by analysing overlapping peptides obtained from partial acid hydrolysates of isolated sacculi. It is suggested that the peptide subunits are attached to glycan strands via one of their glutamyl residues. Another glutamyl residue may crosslink two adjacent peptide subunits to form a dimer. The calculated molar ratios of the amino acids and the percentages of the N-or C-terminal amino acid residues of the supposed dimers are compatible with those actually found in the sacculus polymer.

Key words

Methanobacterium thermoautotrophicum Cell wall Pseudomurein Amino acid sequence 


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  1. Balch, W. E., Magrum, L. J., Fox, G. E., Wolfe, R. S., Woese, C. R.: An ancient divergence among the bacteria. J. Mol. Evol. 9, 305–311 (1977)Google Scholar
  2. Kandler, O.: Zellwandstrukturen bei Methan-Bakterien. Zur Evolution der Prokaryonten. Naturwissenschaften 66, 95–105 (1979)Google Scholar
  3. Kandler, O., König, H.: Chemical composition of the pepdiglycan-free cell walls of methanogenic bacteria. Arch. Microbiol. 118, 141–152 (1978)Google Scholar
  4. Kawanishi, Y., Iwai, K., Ando, T.: Determination of C-terminal arginine and asparagine of proteins by catalytic hydrazinolysis. J. Biochem. 56, 314–324 (1964)Google Scholar
  5. Perkins, H. R.: The use of photolysis of dinitrophenylpeptides in structural studies on the cell-wall mucopeptide of Corynebacterium poinsettiae. Biochem. J. 102, 29c-32c (1967)Google Scholar
  6. Russell, D. W.: Studies on the photochemical behaviour of 2,4-dinitrophenyl derivatives of some amino acids and peptides. Biochem. J. 87, 1–4 (1963)Google Scholar
  7. Schleifer, K. H., Kandler, O.: Peptidoglycan types of bacterial cell walls and their taxonomic implication. Bacteriol. Rev. 36, 407–477 (1972)Google Scholar
  8. Weidl, W.: Ein neuer Typ von Macromolekülen. Angew. Chem. 76, 801–832 (1964)Google Scholar
  9. Weidl, W., Pelzer, H.: Bagshaped macromolecules: A new outlook on bacterial cell walls. Adv. Enzymol. 26, 193–232 (1964)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Helmut König
    • 1
  • Otto Kandler
    • 1
  1. 1.Botanisches Institut der Universität MünchenMünchen 19Germany

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