Archives of Microbiology

, Volume 121, Issue 3, pp 207–211 | Cite as

Separation and identification of the carotenoid pigments of stigmata isolated from light-grown cells of Euglena gracilis strain Z

  • Diana V. Heelis
  • William Kernick
  • Glyn O. Phillips
  • Keith Davies


Thin layer chromatography of the carotenoid pigments of stigmata isolated from light grown cells of Euglena gracilis strain Z resolved 29 compounds, of which 16 could be eluted and their absorption spectra recorded. Seven of these compounds were identified by a combination of co-chromatography with authentic compounds and by chemical tests, one of these compounds (β-carotene) was further identified by mass spectrometry. The major carotenoids were found to be β-carotene, diatoxanthin and diadinoxanthin which together comprised approximately 60% of the stigma pigments. In addition significant quantities of canthaxanthin, echinenone and cryptoxanthin were isolated and a possible carotenoid ester was detected. The results of this analysis are compared with those of previous workers and the significance of the findings is discussed.

Key words

Euglena Stigma Carotenoids TLC 



thin layer chromatography




Light petroleum


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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Diana V. Heelis
    • 1
  • William Kernick
    • 1
  • Glyn O. Phillips
    • 1
  • Keith Davies
    • 2
  1. 1.The North East Wales InstituteKelsterton CollegeClwydUK
  2. 2.University of SalfordSalfordUK

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