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Archives of Microbiology

, Volume 121, Issue 3, pp 207–211 | Cite as

Separation and identification of the carotenoid pigments of stigmata isolated from light-grown cells of Euglena gracilis strain Z

  • Diana V. Heelis
  • William Kernick
  • Glyn O. Phillips
  • Keith Davies
Article

Abstract

Thin layer chromatography of the carotenoid pigments of stigmata isolated from light grown cells of Euglena gracilis strain Z resolved 29 compounds, of which 16 could be eluted and their absorption spectra recorded. Seven of these compounds were identified by a combination of co-chromatography with authentic compounds and by chemical tests, one of these compounds (β-carotene) was further identified by mass spectrometry. The major carotenoids were found to be β-carotene, diatoxanthin and diadinoxanthin which together comprised approximately 60% of the stigma pigments. In addition significant quantities of canthaxanthin, echinenone and cryptoxanthin were isolated and a possible carotenoid ester was detected. The results of this analysis are compared with those of previous workers and the significance of the findings is discussed.

Key words

Euglena Stigma Carotenoids TLC 

Abbreviations

T.L.C.

thin layer chromatography

C

Carotenoid

L.P.

Light petroleum

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References

  1. Bamji, M. S., Krinsky, N. I.: Carotenoid de-epoxidations in algae. II. Enzymatic conversion of antheraxanthin to zeaxanthin. J. Biol. Chem. 240, 467–470 (1965)Google Scholar
  2. Bartlett, C. J., Walne, P. L., Schwarz, O. J., Brown, D. M.: Large scale isolation and purification of eyespot granules from Euglena gracilis var. bacillaris. Plant Physiol. 49, 881–885 (1972)Google Scholar
  3. Batra, P. P., Tollin, G.: Phototaxis in Euglena I. Isolation of the cyespot granules and identification of the eyespot pigments. Biochim. Biophys. Acta 79, 371–378 (1964)Google Scholar
  4. Benedetti, P. A., Checcucci, A.: Paraflagellar body (PFB) pigments studied by fluorescence microscopy in Euglena gracilis. Plant Sci. Letters 4, 47–51 (1975)Google Scholar
  5. Britton, G., Goodwin, T. W.: Biosynthesis of carotenoids. Methods Enzymol. 18, 654–706 (1971)Google Scholar
  6. Cramer, M., Myers, J.: Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384–402 (1952)Google Scholar
  7. Davies, B. H.: The Carotenoids, In: The Chemistry and Biochemistry of Plant Pigments. Vol. 2 (T. W. Goodwin, ed.), pp. 38–165. London: Academic Press 1976Google Scholar
  8. Diehn, B.: Action spectra of the phototactic responses in Euglena. Biochim. Biophys. Acta 177, 136–143 (1969)Google Scholar
  9. Diehn, B.: Sensory transduction in a unicellular organism: Light-induced motor responses of Euglena. Science 181, 1009–1015 (1969)Google Scholar
  10. Goodwin, T. W., Jamikorn, M.: Studies in carotenogenesis. Some observations on carotenoid synthesis in two varieties of Euglena gracilis. J. Protozool. 1, 216–219 (1954)Google Scholar
  11. Heelis, D. V.: Ph. D. thesis, Salford University U.K. (1977)Google Scholar
  12. Krinsky, N. I., Goldsmith, T. H.: The carotenoids of the flagellated-alga, Euglena gracilis. Arch. Biochem. Biophys. 91, 271–279 (1960)Google Scholar
  13. Krinsky, N. I.: A relationship between partition coefficients of carotenoids and their functional groups. Analytical Biochemistry 6, 293–302 (1963)Google Scholar
  14. Krinsky, N. I.: Carotenoid de-epoxidations in algae. I. Photochemical transformation of antheraxanthin to zeaxanthin. Biochim. Biophys. Acta 88, 487–491 (1964)Google Scholar
  15. Kivic, P. A., Vesk, M.: Structure and function in the Euglenoid eyespot apparatus. The fine structure and response to environmental changes. Planta 105, 1–14 (1972)Google Scholar
  16. Kivic, P. A., Vesk, M.: The structure of the eyespot apparatus in bleached strains of Euglena gracilis. Cytobiologie 10, 88–101 (1974)Google Scholar
  17. Lenci, F., Colombetti, G.: Photobehaviour of micro-organisms: A biophysical approach. Ann. Rev. Biophys. Bioeng. 7, 347–361 (1978)Google Scholar
  18. Sperling-Pagni, P. G., Walne, P. L., Wehry, E. L.: Fluorometric evidence for flavins in isolated eyespots of Euglena gracili. var. bacillaris. Photochem. Photobiol. 24, 373–375 (1976)Google Scholar
  19. Strain, H. H.: Molecular structure and adsorption sequences of carotenoid pigments. J. Amer. Chem. Soc. 70, 588–591 (1948)Google Scholar
  20. Walne, P. L., Arnott, H. J.: The comparative and possible function of eyespots: Euglena granulata and Chlamydomonas eugametos. Planta 77, 325–353 (1967)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Diana V. Heelis
    • 1
  • William Kernick
    • 1
  • Glyn O. Phillips
    • 1
  • Keith Davies
    • 2
  1. 1.The North East Wales InstituteKelsterton CollegeClwydUK
  2. 2.University of SalfordSalfordUK

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