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Archiv für Mikrobiologie

, Volume 87, Issue 2, pp 119–128 | Cite as

Rhythmic mycelial growth in Podospora anserina

V. The levels of phosphorylated intermediates
  • Tilmann Bornefeld
  • Gernot Lysek
Article

Summary

  1. 1.

    After 32P-labelling the pool sizes of the phosphorylated intermediates and their changes in the wild strain and in the rhythmically growing mutant zonata of P. anserina were studied by thin-layer chromatography.

     
  2. 2.

    During the growth period of four days, in which the mutant forms the first growth band, the following differences between these two strains were observed:

    1. a)

      The pools of the UDPG and of the hexosephosphates were altered markedly in the wild strain, no such changes occurred in zonata.

       
    2. b)

      In the mutant the ratio between the F-6-P or the entire hexosephosphates, respectively, and the F-di-P was shifted towards the side of the diphosphate.

       
    3. c)

      Simultaneously in this strain the pool of the energy-rich nucleotidetriphosphates was enhanced in relation to the monophosphates.

       
    4. d)

      Zonata accumulated high, amounts of phosphoryl choline and phosphoryl ethanolamine.

       
     
  3. 3.

    It was attempted to correlate these differences to the altered hyphal growth and development of the mutant.

     

Keywords

Chromatography Choline Monophosphates Diphosphate Growth Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

F-di-P

Fructose-1,6-diphosphate

F-6-P

Fructose-6-phosphate

G-6-P

Glucose-6-phosphate

PEP

Phosphoenolpyruvate

PFK

Phosphofructokinase

PGA

Phosphoglyceric acid

Pi

Inorganic phosphate

UDPG

Uridine diphosphoglucose

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References

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Tilmann Bornefeld
    • 1
  • Gernot Lysek
    • 2
  1. 1.Botanisches Institut I der Universität WürzburgGermany
  2. 2.Botanisches Institut der Technischen Universität MünchenFreising-WeihenstephanGermany

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