Morphogenesis of Trichoderma: Autoradiography of intact colonies labeled by [3H]N-acetylglucosamine as a marker of new cell wall biosynthesis
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[3H]N-acetylglucosamine ([3H]-NAGA) was incorporated into intact colonies of Trichoderma viride to follow autoradiographically the localization and timing of extension, initiation of new tips and other changes in the hyphal walls.
It was found that [3H]-NAGA incorporation differed greatly from [3H] uridine and [3H]leucine incorporation as regards labeling pattern and susceptibility to enzymes.
Incorporation for 6, 18 and 54 min resulted in labeling of cell wall regions in fair correspondence to the hyphal growth rate, in conformity with the assumption that chitin synthesis is mainly apical.
Pulse labeling indicated that hyphal tip growth took place both at the colony-margin and in a submarginal ring. The latter labeling was very conscious in photoinduced colonies where conidiophore formation was followed also by “pulse and wash” labeling.
The general applicability of the method to morphogenetic studies in fungal colonies is discussed.
KeywordsChitin Trichoderma Uridine Cell Wall Biosynthesis Pulse Label
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