Archiv für Mikrobiologie

, Volume 86, Issue 4, pp 305–314 | Cite as

Morphogenesis of Trichoderma: Autoradiography of intact colonies labeled by [3H]N-acetylglucosamine as a marker of new cell wall biosynthesis

  • Esra Galun
Article

Summary

  1. 1.

    [3H]N-acetylglucosamine ([3H]-NAGA) was incorporated into intact colonies of Trichoderma viride to follow autoradiographically the localization and timing of extension, initiation of new tips and other changes in the hyphal walls.

     
  2. 2.

    It was found that [3H]-NAGA incorporation differed greatly from [3H] uridine and [3H]leucine incorporation as regards labeling pattern and susceptibility to enzymes.

     
  3. 3.

    Incorporation for 6, 18 and 54 min resulted in labeling of cell wall regions in fair correspondence to the hyphal growth rate, in conformity with the assumption that chitin synthesis is mainly apical.

     
  4. 4.

    Pulse labeling indicated that hyphal tip growth took place both at the colony-margin and in a submarginal ring. The latter labeling was very conscious in photoinduced colonies where conidiophore formation was followed also by “pulse and wash” labeling.

     
  5. 5.

    The general applicability of the method to morphogenetic studies in fungal colonies is discussed.

     

Keywords

Chitin Trichoderma Uridine Cell Wall Biosynthesis Pulse Label 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bartnicki-Garcia, S.: Cell wall chemistry, morphogenesis and taxonomy of fungi. Ann. Rev. Microbiol. 22, 87–108 (1968).Google Scholar
  2. —, Lippman, E.: Fungal morphogenesis: cell wall construction in Mucor rouxii. Science 165, 302–304 (1969).Google Scholar
  3. Cabib, E., Farkas, V.: The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation. Proc. nat. Acad. Sci. (Wash.) 68, 2052–2056 (1971).Google Scholar
  4. Galun, E.: Morphogenesis in Trichoderma: Induction of conidiation by narrow-beam illumination of restricted areas of the fungal colony. Plant & Cell Physiol. 12, 779–783 (1971a).Google Scholar
  5. —: Scanning electron microscopic studies of intact Trichoderma colonies. J. Bact. 108, 938–940 (1971b).Google Scholar
  6. —, Gressel, J.: Morphogenesis in Trichoderma: suppression of photoinduction by 5-fluorouracil. Science 151, 696–698 (1966).Google Scholar
  7. Girbardt, M.: Die Ultrastruktur der Apikalregion von Pilzhyphen. Protoplasma (Wien) 67, 413–441 (1969).Google Scholar
  8. Gressel, J., Galun, E.: Morphogenesis in Trichoderma: photoinduction and RNA. Develop. Biol. 15, 575–598 (1967).Google Scholar
  9. —, Hartmann, K.: Morphogenesis in Trichoderma: action spectrum of photoinduced sporulation. Planta (Berl.) 79, 271–274 (1968).Google Scholar
  10. —, Strausbauch, L., Galun, E.: Morphogenesis in Trichoderma: Photomimetic effect of acetylcholine. Nature (Lond.) 232, 648–649 (1971).Google Scholar
  11. Grove, S. N., Bracker, C. E.: Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkörper. J. Bact. 104, 989–1009 (1970).Google Scholar
  12. Katz, D., Rosenberger, R. F.: Hyphal wall synthesis in Aspergillus nidulans: effect of protein synthesis inihibition and osmotic shock on chitin insertion and morphogenesis. J. Bact. 108, 184–190 (1971a).Google Scholar
  13. ——: Lysis of an Aspergillus nidulans mutant blocked in chitin synthesis and its relation to wall assembly and wall metabolism. Arch. Mikrobiol. 80, 284–292 (1971b).Google Scholar
  14. Stavy (Rodeh), R., Galun, E., Gressel, J.: Morphogenesis in Trichoderma: RNA-DNA hybridization studies. Biochim. biophys. Acta (Amst.) 259, 321–329 (1972).Google Scholar
  15. Troy, F. A., Koffler, H.: The chemistry and molecular architecture of the cell walls of Penicillium chrysogenum. J. biol. Chem. 244, 5563–5576 (1969).Google Scholar
  16. Weiman-Greensphan, D., Galun, E.: Complementation in non-conidiating mutants of Trichoderma. J. Bact. 99, 802–806 (1969).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Esra Galun
    • 1
  1. 1.Plant Genetics DepartmentThe Weizmann Institute of ScienceRehovotIsrael

Personalised recommendations