Archiv für Mikrobiologie

, Volume 79, Issue 4, pp 354–366 | Cite as

Inhibition of growth of obligately chemolithotrophic thiobacilli by amino acids

  • May C. Lu
  • Abdul Matin
  • Sydney C. Rittenberg


  1. 1.

    The effects of eighteen l-amino acids and two peptides, added individually to an otherwise autotrophic medium, on the growth of Thiobacillus thioparus, Thiobacillus neapolitanus and Thiobacillus thiooxidans were tested. Under these conditions, specific l-amino acids, which differed for each of the thiobacilli, inhibited growth.

  2. 2.

    All inhibitory effects were reversed, completely or in part, in the presence of non-inhibitory concentrations of casein hydrolysate.

  3. 3.

    l-tyrosine or l-tryptophane reversed the l-phenylalanine inhibition of T. thioparus and T. neapolitanus; l-serine inhibition of T. thiooxidans was partially reversed by l-threonine and completely by l-valine plus l-leucine; and l-valine inhibition of T. thiooxidans was similarly reversed by l-leucine or l-isoleucine.

  4. 4.

    In cell-free systems derived from T. thiooxidans, l-valine or l-isoleucine inhibited the activity of acetolactate-forming enzyme. Repression of synthesis of this enzyme by l-valine, l-isoleucine and l-leucine could not be demonstrated.

  5. 5.

    d-valine and d-isoleucine were also inhibitory to T. thioparus. The inhibition by d-valine was not reversed in the presence of any combination of the l-isomers of valine, isoleucine and leucine.

  6. 6.

    It is concluded that inhibition of growth of these three obligately chemolithotrophic thiobacilli by l-amino acids is due to amino acid imbalances that derange normal regulatory processes as also occurs in chemoorganotrophic bacteria.



Casein Hydrolysate Thiobacillus Amino Acid Imbalance Chemoorganotrophic Bacterium Thiobacillus Thiooxidans 
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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • May C. Lu
    • 1
  • Abdul Matin
    • 1
  • Sydney C. Rittenberg
    • 1
  1. 1.Department of BacteriologyUniversity of CaliforniaLos AngelesUSA

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