Archiv für Mikrobiologie

, Volume 88, Issue 2, pp 119–126 | Cite as

Conidiation of Aspergillus niger in continuous culture

  • A. M. L. Ng
  • J. E. Smith
  • A. F. McIntosh
Article

Summary

Conidiation of Aspergillus niger was studied in carbon-limited and nitrogen-limited chemostat culture. Under citrate-limitation conidiation intensity varied inversely with dilution rate. Conidiophores were less complex than in aerial conidiation and at high dilution rates conidia occasionally developed from modified hyphal tips. Conidiation was difficult to achieve under glucose-limitation. At the low dilution rates that allowed limited conidiation steady state could not be maintained due to onset of autolysis. At higher dilution rates when steady state was readily obtained conidiation did not occur. The maximum yield constants under citrate-limitation and glucose-limitation were respectively 0.145 and 0.4 mg dry weight/mg substrate, while the relative specific maintenance values were 0.045 and 0.018 mg substrate/mg dry weight/h. Under ammonium-limitation with citrate as the carbon source there was no conidiation. When nitrate became the limiting nitrogen source conidiophore initiation occurred but biomass production was low and wash-out occurred at D=0.034 h-1.

Keywords

Biomass Steady State Aspergillus Nitrogen Source Biomass Production 

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References

  1. Anderson, J. G., Smith, J. E.: Synchronous initiation and maturation of Aspergillus niger conidiophores in culture. Trans. Brit. Mycol. Soc. 56, 9–19 (1971a).Google Scholar
  2. Anderson, J. G., Smith, J. E.: The production of conidiophores and conidia by newly germinated conidia of Aspergillus niger (microcycle conidiation). J. gen. Microbiol. 69, 185–197 (1971b).Google Scholar
  3. Carter, B. L. A., Bull, A. T.: The effect of oxygen tension in the medium on the morphology and growth kinetics of Aspergillus nidulans. J. gen. Microbiol. 65, 265–273 (1971).Google Scholar
  4. Conway, E. J.: Microdiffusion analysis and volumetric error. Edinburgh: Crosby Lockwood 1957.Google Scholar
  5. Dawes, I. W., Mandelstam, J.: Sporulation of Bacillus subtilis in continuous culture. J. Bact. 103, 529–535 (1970).Google Scholar
  6. El Kotry, R. A. R.: Physiological studies on the production of amylase by Aspergillus oryzae in batch and continuous cultures. Ph.D. Thesis, Strathclyde University, Glasgow 1970.Google Scholar
  7. Galbraith, J. C., Smith, J. E.: Sporulation of Aspergillus niger in submerged liquid culture. J. gen. Microbiol. 59, 31–45 (1969).Google Scholar
  8. Hawker, L. E.: Environmental influences on reproduction. In: The fungi, Vol. 11, pp. 435–469, ed. by G. C. Ainsworth and A. S. Sussman. New York-London: Academic Press Inc. 1966.Google Scholar
  9. Mandelstam, J.: Regulation of bacterial spore formation. Symp. Soc. J. Microbiol. 19, 377–402 (1969).Google Scholar
  10. Morton, A. G.: The induction of sporulation in mould fungi. Proc. roy. Soc. B 153, 548–569 (1961).Google Scholar
  11. Ng, W. S., Smith, J. E., Anderson, J. G.: Changes in carbon catabolic pathways during synchronous development of conidiophores of Aspergillus niger. J. gen. Microbiol. 71, 495–504 (1972).Google Scholar
  12. Pirt, S. J., Callow, D. S.: Studies on the growth of Penicillium chrysogenum in continuous flow culture with reference to penicillin production. J. appl. Bact. 23, 87–98 (1960).Google Scholar
  13. Righelato, R. C., Trinci, A. P. J., Pirt, S. J., Peat, A.: The influence of maintenance energy and growth rate on the metabolic activity, morphology and conidiation of Penicillium chrysogenum. J. gen. Microbiol. 50, 399–412 (1968).Google Scholar
  14. Saffran, M., Denstedt, O. F.: A rapid method for the determination of citric acid. J. biol. Chem. 175, 849–855 (1948).Google Scholar
  15. Schulze, K. L., Lipe, R. S.: Relationship between substrate concentration, growth rate, and respiration rate of Escherichia coli in continuous culture. Arch. Mikrobiol. 48, 1–20 (1964).Google Scholar
  16. Smith, J. E., Galbraith, J. C.: Biochemical and physiological aspects of differentiation in the fungi. Advanc. Microbiol. Physiol. 5, 45–134 (1971).Google Scholar
  17. Smith, J. E., Ng, W. S.: Fluorometric determination of glycolytic intermediates and adenylates during sequential changes in replacement culture of Aspergillus niger. Canad. J. Microbiol. (in press, 1972).Google Scholar
  18. Smith, J. E., Valenzuela-Perez, J.: Changes in intracellular concentrations of glycolytic intermediates and adenosine phosphates during growth cycle of Aspergillus niger. Trans. Brit. Mycol. Soc. 57, 103–110 (1971).Google Scholar
  19. Smith, J. E., Valenzuela-Perez, J., Ng, W. S.: Changes in activities of the Embden-Meyerhof-Parnas and pentose phosphate pathways during the growth cycle of Aspergillus niger. Trans. Brit. Mycol. Soc. 57, 93–101 (1971).Google Scholar
  20. Turian, G.: Différenciation fongique. Paris: Massay and Co. 1969.Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • A. M. L. Ng
    • 1
  • J. E. Smith
    • 1
  • A. F. McIntosh
    • 1
  1. 1.Department of Applied MicrobiologyStrathclyde UniversityGlasgowScotland

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