Archiv für Mikrobiologie

, Volume 88, Issue 2, pp 119–126 | Cite as

Conidiation of Aspergillus niger in continuous culture

  • A. M. L. Ng
  • J. E. Smith
  • A. F. McIntosh


Conidiation of Aspergillus niger was studied in carbon-limited and nitrogen-limited chemostat culture. Under citrate-limitation conidiation intensity varied inversely with dilution rate. Conidiophores were less complex than in aerial conidiation and at high dilution rates conidia occasionally developed from modified hyphal tips. Conidiation was difficult to achieve under glucose-limitation. At the low dilution rates that allowed limited conidiation steady state could not be maintained due to onset of autolysis. At higher dilution rates when steady state was readily obtained conidiation did not occur. The maximum yield constants under citrate-limitation and glucose-limitation were respectively 0.145 and 0.4 mg dry weight/mg substrate, while the relative specific maintenance values were 0.045 and 0.018 mg substrate/mg dry weight/h. Under ammonium-limitation with citrate as the carbon source there was no conidiation. When nitrate became the limiting nitrogen source conidiophore initiation occurred but biomass production was low and wash-out occurred at D=0.034 h-1.


Biomass Steady State Aspergillus Nitrogen Source Biomass Production 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • A. M. L. Ng
    • 1
  • J. E. Smith
    • 1
  • A. F. McIntosh
    • 1
  1. 1.Department of Applied MicrobiologyStrathclyde UniversityGlasgowScotland

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