Zusammenfassung
Zellfreier Extrakt von Clostridium butyricum kann die phosphoroclastische Reaktion und deren Umkehrung, die reduktive Carboxylierung von Acetylphosphat zu Brenztraubensäure, katalysieren. Durch die Behandlung an einer DEAE-Cellulosesäule wird das Ferredoxin vom Extrakt separiert; der Extrakt verliert dabei seine enzymatische Wirksamkeit. Durch Rekombination werden bis zu 40% der ursprünglichen Enzymaktivität wiederhergestellt. Aus dem gebildeten Pyruvat werden, besonders in Anwesenheit von Diphosphopyridinnucleotid, Glutamin und Mangan, weitere Verbindungen synthetisiert.
Summary
Cellfree extracts of Cl. butyricum catalyze the phosphoroclastic cleavage and the reversal of the reaction, the reductive carboxylation of acetylphosphate to pyruvate. Treatment of the extracts with DEAE-Cellulose removes the ferredoxin and the extract looses its catalytic activity. Adding back ferredoxin to DEAE-treated extracts restores the activity for synthesizing pyruvate up to 40%. Pyruvate serves as substrate for various reactions, which can be stimulated by DPN, Mn, and an amino donor.
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Abbreviations
- Fd:
-
Ferredoxin
- TPP:
-
Thiaminpyrophosphat
- ATP:
-
Adenosintriphosphat
- DPN:
-
Diphosphopyridinnucleotid (NAD)
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Heer, E., Bachofen, R. Pyruvatstoffwechsel von Clostridium butyricum. Archiv. Mikrobiol. 54, 1–13 (1966). https://doi.org/10.1007/BF00424704
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DOI: https://doi.org/10.1007/BF00424704