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Identification and determination of pyrene and benzo(a)pyrene quinones by means of fluorescence spectrography at — 197°C. application to air pollution analysis

Nachweis und Bestimmung von Pyren- und, Benz(a)pyrenchinonen durch Fluorescenzspektrographie bei — 197°C. Anwendung zur Analyse von Luftverunreinigungen

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Zusammenfassung

Die Verbindungen werden zunächst gemeinsam von anderen Substanzen durch Dünnschicht-Chromatographie auf Al2O3 [mit Toluol/Äther (3∶2)] und anschließend voneinander auf Silicagel [mit Benzol/Aceton (9∶1)] getrennt. Die Bestimmung erfolgt durch Fluorescenzmessung der isolierten Verbindungen bei − 197°C. Hierbei wird die photometrische Reaktion der alkoholischen Lösung der Chinone ausgenutzt, bei der stark fluorescierende Produkte gebildet werden. Die Nachweisgrenzen liegen für Pyrenchinone bei 0,3 μg/ml, für Benz(a)pyrenchinone bei 0,5 μg/ml. In der Atmosphäre von Prag konnten nur Spuren von Benz(a)pyrenchinonen festgestellt werden.

Summary

A method for the identification and determination of pyrene and benzo(a)pyrene quinones based on the successive thin-layer chromatographic separation on Al2O3 [with toluene/ether (3∶2)] and silica gel [with benzene/acetone (9∶1)] and fluorescence measurement of the isolated substances. The photochemical reaction of pyrene and benzo(a)pyrene quinones in their ethanolic solutions, by which intensively fluorescent products are formed, was utilized. The sensitivity and selectivity of the estimation is further enhanced by recording fluorescence spectra of these substances at the temperature of the boiling point of liquid nitrogen. The limits of detection for pyrene and benzo(a)pyrene quinones are 0.3 μg/ml and 0.5 μg/ml, respectively. Prague atmosphere contains only trace amounts of benzo(a)pyrene quinones and does not contain detectable amounts of pyrene quinones.

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Authors and Affiliations

  1. Institute of Hygiene and Epidemiology, Prague, Czech Republic

    Jiří Jäger

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  1. Jiří Jäger
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Jäger, J. Identification and determination of pyrene and benzo(a)pyrene quinones by means of fluorescence spectrography at — 197°C. application to air pollution analysis. Z. Anal. Chem. 255, 281–284 (1971). https://doi.org/10.1007/BF00424196

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  • DOI: https://doi.org/10.1007/BF00424196

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