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Spectrophotometric determination of vitamin C in citrus fruits using peri-naphthindan-2,3,4-trione

Spektralphotometrische Vitamin C-Bestimmung in Citrusfrüchten unter Verwendung von peri-Naphthindan-2,3,4-trion

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Zusammenfassung

Das Verfahren, das auf Citronen-, Orangen- und Grapefruit-Säfte angewendet wurde, beruht auf der Reaktion mit peri-Naphthindan-2,3,4-trion bei pH 3. Hierbei wird Dihydroxy-peri-naphthindenon gebildet, das in methanolischer Lösung bei 460 nm gemessen wird. Die Methode weist eine befriedigende Genauigkeit auf und die Ergebnisse sind denen der titrimetrischen U.S.P.-Methode ebenbürtig. Dehydroascorbinsäure sowie andere Saftbestandteile verursachen keine Störungen.

Summary

A spectrophotometric method for the determination of vitamin C in lemon, orange, and grapefruit juices is described. It is based on the reaction with peri-naphthindan-2,3,4-trione hydrate at pH 3 to give dihydroxy-peri-naphthindenone which is dissolved in methanol and measured at 460 nm. The method shows good precision and its accuracy compares favourably with the standard titrimetric procedure of the U.S.P. No interferences are caused by dehydroascorbic acid as well as many other juice ingredients.

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Author notes

  1. M. M. Abd El Fattah

    Present address: Max-Planck-Institut für Biochemie, D-8033, Martinsried bei München

Authors and Affiliations

  1. Research Microanalytical Laboratory, Departments of Chemistry and Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt

    Saad S. M. Hassan, M. M. Abd El Fattah & M. T. M. Zaki

Authors
  1. Saad S. M. Hassan
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  2. M. M. Abd El Fattah
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  3. M. T. M. Zaki
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Hassan, S.S.M., El Fattah, M.M.A. & Zaki, M.T.M. Spectrophotometric determination of vitamin C in citrus fruits using peri-naphthindan-2,3,4-trione. Z. Anal. Chem. 277, 369–371 (1975). https://doi.org/10.1007/BF00423782

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  • DOI: https://doi.org/10.1007/BF00423782

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