Antonie van Leeuwenhoek

, Volume 52, Issue 6, pp 555–566 | Cite as

Influence of light and natural microbiota of the Butrón river on E. coli survival

  • I. Barcina
  • I. Arana
  • J. Iriberri
  • L. Egea


The survival of an E. coli strain in water samples from the Butrón river has been studied. The input of E. coli cells in the aquatic system breaks down the established balance among the components of the natural microbiota: E. coli becomes the object of the active protozoal predation whereas the autochtonous heterotrophic community become alternative preys. As a result of this new situation, the natural microbiota increases but returns to the initial values once the E. coli cells have been removed from the system. The effect of the temperature of incubation on the survival is exerted through the effect of this parameter on the predatory activity of the protozoa. Light has a lethal and direct action on the E. coli cells, the effect of this parameter is even superior to that of predation.

Key words

E. coli survival freshwater natural microbiota light 


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  1. Anderson, J. C., Rhodes, M. W. & Kator, K. I. (1983) Seasonal variation in survival of E. coli exposed ‘in situ’ membrane diffusion chambers containing filtered and non filtered estuarine water. Appl. Envir. Microbiol. 45: 1877–1883Google Scholar
  2. Cabridence, R. & Lepailleur, H. (1969) Rôle des alges dans l'épuration biologique des eaux. Terres et eaux 58: 12–18Google Scholar
  3. Costerton, J. W. & Colwell, R. R. (1979) Native aquatic bacteria: enumeration, activity and ecology. ASTM special technical publication 695. Costerton & Colwell Ed.Google Scholar
  4. Danso, S. K. A. & Alexander, M. (1975) Regulation of predation by prey density: the protozoan-Rhizobium relationship. Appl. Microbiol. 29: 515–521Google Scholar
  5. Davenport, C. V., Sparrow, E. B. & Gordon, R. C. (1976) Fecal indicator bacteria persistence under natural conditions in an ice-covered river. Appl. Envir. Microbiol. 32: 527–536Google Scholar
  6. Dive, D. (1973) La nutrition holozoîque des protozoaires ciliés. Ses consequences dans l'épuration naturelle et artificielle. A. N. N. Biol. 12: 343–380Google Scholar
  7. Enzinger, R. M. & Cooper, R. C. (1976) Role of bacteria and protozoa in the removal of E. coli from estuarine waters. Appl. Envir. Microbiol. 31: 758–763Google Scholar
  8. Gameson, A. L. H. (1984) Investigations of sewage discharges to some british coastal waters. Bacterial mortality, 1. Wat. Res. Cent.: 1–34Google Scholar
  9. Grigsby, P. & Calkins, J. (1979) The inactivation of a natural population of coliform bacteria by sunlight. Photochem. Photobiol. 31: 291–294Google Scholar
  10. Habte, M. & Alexander, M. (1978) Protozoan density and the coexistence of protozoan predator and bacterial prey. Ecology 59: 140–146Google Scholar
  11. Jagger, J. (1975) Inhibition by sunlight of the growth of E. coli B/r. Photochem. Photobiol. 22: 67–70Google Scholar
  12. Jannasch, H. W. (1968) Competitive elimination of Entero-bacteriaceae from seawater. Appl. Microbiol. 16: 1616–1618Google Scholar
  13. Kapuscinski, R. B. & Mitchell, R. (1981) Solar radiation induces sublethal injury in E. coli in sea water. Appl. Microbiol. 41: 670–674Google Scholar
  14. Kittrell, F. W. & Furfari, S. A. (1963) Observations of coliform bacteria in streams. J. Wat. Pollut. Cont. Fed. 35: 1361–1385Google Scholar
  15. Lechevalier, M. W. & McFeters, G. A. (1985) Interactions between heterotrophic plate count bacteria and coliform organisms. Appl. Envir. Microbiol. 49: 1338–1341Google Scholar
  16. McCambridge, J. & McMeekin, T. A. (1979) Protozoan predation of E. coli in estuarine waters. Wat. Res. 13: 659–663Google Scholar
  17. McCambridge, J. & McMeekin, T. A. (1980) Relative effects of bacterial and protozoan predators on survival of E. coli in estuarine water samples. Appl. Envir. Microbiol. 40: 907–911Google Scholar
  18. McCambridge, J. & McMeekin, T. A. (1981) Effect of solar radiation and predacious microorganisms on survival of fecal and other bacteria. Appl. Envir. Microbiol. 41: 1083–1087Google Scholar
  19. McFeters, G. A. & Stuart, D. G. (1972) Survival of coliform bacteria in natural waters: field and laboratory studies with membrane-filters chambers. Appl. Microbiol. 24: 805–811Google Scholar
  20. Saz, A. K., Watson, S., Brown, S. R. & Lowery, D. C. (1963) Antimicrobial activity of marine waters. Macromolecular nature of antistaphylococcae factor. Limnol. Oceanogr. 8: 63–66Google Scholar
  21. Sieburth, J. McN. & Pratt, D. M. (1962) Anticoliform activity of sea water associated with the termination of Skeletonema costatum blooms. Trans. N. Y. Acad. Sci. 24: 495–501Google Scholar
  22. Singh, B. N. (1955) Culturing soil protozoa and estimating their numbers in soil. In: D. K. M. Kevan (ed), Soil zoology. Butterworths Scientific Publications, LondonGoogle Scholar
  23. Stolp, H. & Starr, M. P. (1963) Bdellovibrio bacteriovorous gen. et sp. n., a predatory, ectoparasitic and bacteriolytic microorganism. Antonie van Leeuwenhoek 29: 217–248Google Scholar
  24. Taylor, W. D. (1978) Growth responses of ciliate protozoa to the abundance of their bacterial prey. Microbiol. Ecology 4: 207–214Google Scholar
  25. Verstraete, W. & Voets, J. P. (1976) Comparative study of E. coli survival in two aquatic ecosystems. Wat. Res. 10: 129–136Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • I. Barcina
    • 1
  • I. Arana
    • 1
  • J. Iriberri
    • 1
  • L. Egea
    • 1
  1. 1.Departmento de Biologia, Facultad de CienciasUniversidad del Pais VascoBilbaoSpain

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