Abstract
A mass fragmentographic method for the simultaneous determination of 4-hydroxy-3-methoxyphenylacetic acid (HVA), 4-hydroxy-3-methoxyphenylethylene glycol (MOPEG), and 5-hydroxyindole-3-acetic acid (5-HIAA) was described. Deuterated analogues of the compounds were used as internal standards. The specificity was proved by multiple ion analysis. The experimental error was below 7% when applied to the analysis of human lumbar cerebrospinal fluid, urine, or rat brain tissue. In cerebrospinal fluid the major part of the monoamine metabolites occurred in the free form. In rat brain and human urine considerable amounts of conjugated HVA was found.
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Beck, O., Sedvall, G.: The synthesis of 5-hydroxyindole-3-(-2-D2-acetic acid) and α,α′,β,β′-D4-5-hydroxytryptamine. J. Lab. Comp. 11, 57–61 (1975)
Bertilsson, L.: Quantitative determination of 4-hydroxy-3-methoxyphenyl glycol and its conjugates in cerebrospinal fluid by mass fragmentography. J. Chromatogr. 87, 147–153 (1973)
Bertilsson, L., Atkinson, A. J., Althaus, J. R., Härfast, Å., Lindgren, J.-E., Holmstedt, B.: Quantitative determination of 5-hydroxyindole-3-acetic acid in cerebrospinal fluid by gas chromatography-mass spectrometry. Analyt. Chem. 44, 1434–1438 (1972)
Fri, C.-G., Wiesel, F.-A., Sedvall, G.: Simultaneous quantification of homovanillic acid and 5-hydroxyindole-acetic acid in cerebrospinal fluid by mass fragmentography. Life Sci. 14, 2469–2480 (1974a)
Fri, C.-G., Wiesel, F.-A., Sedvall, G.: Mass fragmentographic analysis of homovanillic acid and its homoiso analogue in cerebrospinal fluid using the α-dideutero acid as internal standard. Psychopharmacologia (Berl.) 35, 295–304 (1974b)
Gottfries, C. G., Gotfries, J., Roos, B.-E.: Homovanillic acid and 5-hydroxyindolacetic acid in the cerebrospinal fluid of patients with senile dementia, presenile dementia and parkinsonism. J. Neurochem. 16, 1341–1345 (1969)
Karoum, F., Lefèvre, H., Bigelow, L. B., Costa, E.: Urinary excretion of 4-hydroxy-3-methoxyphenylglycol and 4-hydroxy-3-methoxyphenylethanol in man and rat. Clin. chim. Acta 43, 127–137 (1973)
Kirschberg, G. J., Cote, L. J., Lowe, J. H., Ginsburg, S.: Interference with the fluorimetric assay for homovanillic acid caused by acid metabolites of catecholamines. J. Neurochem. 19, 2873–2876 (1972)
Maj, J.: The influence of dopaminergic agents on serotonin neurons. In: Antipsychotic drugs: Pharmacodynamics and pharmacokinetics. G. Sedvall, B. Uvnäs, and Y. Zotterman, eds. London: Pergamon Press 1976
Meek, J. L., Neff, N. H.: Biogenic amines and their metabolites as substrates for phenol sulphotransferase (EC 2.8.2.1) of brain and liver. J. Neurochem. 21, 1–9 (1973)
Persson, T.: Catecholamine turnover in central nervous system. Thesis, University of Gothenburg (1970)
Schanberg, S. M., Schildkraut, J. J., Breese, G. R., Kopin, I. J.: Metabolism of normetanephrine-H3 in rat brain—identification of conjugated 3-methoxy-4-hydroxyphenylglycol as the major metabolite. Biochem. Pharmacol. 17, 247–254 (1968)
Sedvall, G.: Receptor feed-back and dopamine turnover in the central nervous system. In: Handbook of psychopharmacology, L. L. Iversen, S. D. Iversen, and S. H. Snyder, eds., Vol. 6. New York: Plenum Publ. Corp. 1975
Sjöquist, B., Änggård, E.: Gas chromatographic determination of homovanillic acid in human cerebrospinal fluid by electron capture detection and by mass fragmentography with a deuterated internal standard. Analyt. Chem. 44, 2297–2301 (1972)
Sjöquist, B., Lindström, B., Änggård, E.: Mass fragmentographic determination of 4-hydroxy-3-methoxyphenylglycol (HMPG) in urine, cerebrospinal fluid, plasma and tissues using a deuterium-labelled internal standard. J. Chromatogr. 105, 309–316 (1975)
Svensson, T. H., Bunney, B. S., Aghajanian, G. K.: Inhibition of both noradrenergic and serotonergic neurons in brain by the α-adrenergic agonist clonidine. Brain Res. 92, 291–306 (1975)
Wilk, S., Green, J. P.: On the measurement of 5-hydroxyindoleacetic acid in cerebrospinal fluid. J. Neurochem. 19, 2893–2895 (1972)
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Swahn, CG., Sandgärde, B., Wiesel, FA. et al. Simultaneous determination of the three major monoamine metabolites in brain tissue and body fluids by a mass fragmentographic method. Psychopharmacology 48, 147–152 (1976). https://doi.org/10.1007/BF00423253
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DOI: https://doi.org/10.1007/BF00423253