Abstract
The question about the nature of promoters in the transcriptional units containing SV-40 sequences in transformed cells was analyzed. It was found that the pulse-labeled RNA hybridizing to SV-40 DNA contains small but significant amounts of triphosphorylated 5′-ends detected as pppGp in alkaline hydrolyzates of this RNA. In another series of experiments the fragments of RNA containing triphosphorylated 5′-ends about 100 nucleotides in length have been isolated by hydroxyapatite chromatography. Some of them form hybrids with SV-40 DNA. The conclusion is drawn that at least some of SV-40 promoters are used for transcription initiation in SV-40 transformed cells.
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Georgiev, G.P., Samarina, O.P. & Mantieva, V.L. The existence of triphosphorylated 5′-ends in virus-specific RNA isolated from SV-40 transformed cells. Mol Biol Rep 3, 157–165 (1976). https://doi.org/10.1007/BF00423230
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DOI: https://doi.org/10.1007/BF00423230