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Circular dichroism of DNA-proflavine, DNA-ethidium and DNA-distamycine flow-oriented complexes

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Abstract

The circular dichroism (CD) spectra of flow-oriented complexes of DNA with proflavine (PF), ethidium (ET) and distamycine (DS) have been studied in the ultraviolet region. The CD spectra with light propagating in parallel to the flow direction were measured by the method of Chung and Holzwarth [1]. Δ∈ and Δ∈ values have been obtained by this method. It was shown that all the complexes studied exhibit a strong CD anisotropy so that ‘isotropic’ CD spectra measured with a conventional procedure can be attributed to the mutual compensation of the two components of opposite signs.

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References

  1. Chung, S.-X. and Holzwarth, G., J. Mol. Biol. 92, 449–466 (1975).

    Google Scholar 

  2. Yamaoka, K. and Resnik, R., J. Chem. Phys. 70, 4051–4066 (1966).

    Google Scholar 

  3. Dalgleish, D. G., Fugita, H., and Peacocke, A. R., Biopolymers 11, 2415–2422 (1969).

    Google Scholar 

  4. Dalgleish, D. G., Peacocke, A. R., Fey, G., and Harvey, C., Biopolymers 10, 1853–1863 (1971).

    Google Scholar 

  5. Jakson, K and Mason, S. F., Trans. Farad. Soc. 67, 966 (1971).

    Google Scholar 

  6. Poletayev, A. I., Abstr. of IV Intern. Biophys. Congr. EVII, b 4/4 (1972).

  7. Aktipis, S. and Kindelis, A., Biochemistry 12, 1213–1220 (1973).

    Google Scholar 

  8. Zasedatelev, A. S., Gursky, G. V., Zimmer, Ch., and Thrum, H., Mol. Biol. Reports 1, 337–342 (1974).

    Google Scholar 

  9. Parodi, S., Kendall, F., and Nicolini, C., Nucleic Acid Res. 2, 477–486 (1975).

    Google Scholar 

  10. Cohen, G. and Eisenberg, H., Biopolymers 8, 45 (1969).

    Google Scholar 

  11. Moroshina, E. B., Shishov, A. K., Krivtsova, M. A., Zshadin, N. N., and Frisman, E. V., Molek. Biol. (U.S.S.R.) 9, 836 (1975).

    Google Scholar 

  12. Lerman, L. S., J. Mol. Biol. 3, 18 (1961).

    Google Scholar 

  13. Gursky, G. V., Biophysika (U.S.S.R.) 11, 737–746 (1966).

    Google Scholar 

  14. Krey, A. K. and Han, F. E., FEBS Letters 10, 175–178 (1970).

    Google Scholar 

  15. Zasedatelev, A. S., Gursky, G. V., Grochovsky, S. Ya., Zshuze, A. L., Tumanyan, V. G., Zimmer, Ch., and Gottich, B. P., Nature (1976) in press.

  16. Fuller, W. and Waring, M. J., Ber. Bunsenges. Physik. Chem. 68, 805–808 (1964).

    Google Scholar 

  17. Blake, A. and Peacocke, A. R., Biopolymers 6, 1255 (1968).

    Google Scholar 

  18. Waring, M. J., in The Molecular Basis of Antibiotic Action, John Wiley and Sons, London-New York-Sydney-Toronto.

  19. Chung-Che-Tsai, Shri, G., and Jain and Henry M. Sobell, Proc. Nat. Acad. Sci. U.S.A. 72, 628–632 (1975).

    Google Scholar 

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Poletayev, A.I., Minchenkova, L.E. & Zavriev, S.K. Circular dichroism of DNA-proflavine, DNA-ethidium and DNA-distamycine flow-oriented complexes. Mol Biol Rep 3, 131–138 (1976). https://doi.org/10.1007/BF00423226

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  • DOI: https://doi.org/10.1007/BF00423226

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