Skip to main content
SpringerLink
Log in

A nucleolus-specific phosphoprotein in mouse ascites tumor cells

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Analysis of proteins in nucleoli and chromatin of mouse ascites tumor cells labeled with [32P]orthophosphate by SDS-polyacrylamide gel electrophoresis showed that a highly radioactive protein was localized in the nucleoli. This protein was purified and the final preparation appeared as a single component on hydroxylapatite column chromatography with or without SDS. This protein was found to be a nucleolus-specific phosphoprotein with a molecular weight of 120 000. The phosphate moiety in this protein turned over very rapidly whereas the protein itself was stable. When the nucleoli were disrupted by EDTA treatment, this unique protein was found as a major protein constituent of the ultracentrifugal supernatant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kawashima, K., Izawa, M., and Sato, S., Biochim. Biophys. Acta 232, 192 (1971).

    Google Scholar 

  2. Wu, R. S. and Warner, J. R., J. Cell Biol. 51, 643 (1971).

    Google Scholar 

  3. Bachellerie, J. P., Martin-Prevel, C., and Zalta, J. P., Expt. Cell Res. 71, 177 (1972).

    Google Scholar 

  4. Grummt, I., FEBS Letters 39, 125 (1974).

    Google Scholar 

  5. Grummt, I. and Grummt, F., FEBS Letters 39, 129 (1974).

    Google Scholar 

  6. Kang, Y. J., Olson, M. O. J. and Busch, H., J. Biol. Chem. 249, 5580 (1974).

    Google Scholar 

  7. Olson, M. O. J., Orrick, L. R., Jones, C., and Busch, H., J. Biol. Chem. 249, 2823 (1974).

    Google Scholar 

  8. Prestayko, A. W., Olson, M. O. J., and Busch, H., FEBS Letters 44, 131 (1974).

    Google Scholar 

  9. Izawa, M. and Kawashima, K., Biochim. Biophys. Acta 190, 139 (1969).

    Google Scholar 

  10. Weber, K. and Osborn, M., J. Biol. Chem. 244, 4406 (1969).

    Google Scholar 

  11. Moss, B. and Rosenblum, E. N., J. Biol. Chem. 247, 5194 (1972).

    Google Scholar 

  12. Graziano, S. L. and Huang, R. C. C., Biochemistry 10, 4770 (1971).

    Google Scholar 

  13. Bhorjee, J. S. and Pederson, T., Proc. Nat. Acad. Sci. U.S.A. 69, 3345 (1972).

    Google Scholar 

  14. Kleinsmith, L. J., Allfrey, V. G., and Mirsky, A. E., Proc. Nat. Acad. Sci. U.S.A. 55, 1182 (1966).

    Google Scholar 

  15. Kish, V. M. and Kleinsmith, L. J., J. Biol. Chem. 249, 750 (1974).

    Google Scholar 

  16. Langan, T. A.: in A. San Pietro, M. R. Lamborg and F. T. Kenney (eds.), Regulatory Mechanisms for Protein Synthesis in Mammalian Cells, Academic Press, New York, 1968, pp. 101–118.

    Google Scholar 

  17. Allerton, S. E. and Perlmann, G. E., J. Biol. Chem. 240, 3892 (1965).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology Division, National Cancer Center Research Institute, Tsukiji 5-1, Chuo-ku, 104, Tokyo, (Japan)

    K. Kawashima & M. Izawa

Authors
  1. K. Kawashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Izawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawashima, K., Izawa, M. A nucleolus-specific phosphoprotein in mouse ascites tumor cells. Mol Biol Rep 3, 113–119 (1976). https://doi.org/10.1007/BF00423224

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00423224

Keywords

Search

Navigation