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Nuclear and polyribosomal ribonucleoprotein particles containing poly(A) in rat liver cells

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Abstract

Poly(A) containing ribonucleoprotein particles were prepared from rat liver nuclei and polyribosomes. The particles have sedimentation coefficients of 14 S and 9 S, respectively. In Cs2SO4 density gradients the particles banded at densities of 1.28–1.29 g cm-3. Both nuclear and polyribosomal poly(A)-RNP contain in addition to some minor polypeptides, two main polypeptides having molecular weights of 63 000 and 90 000 dalton, respectively indistinguishable from each other according to their electrophoretic mobilities.

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Abbreviations

STKM:

0.25 M sucrose, 0.05 M Tris-HCl, pH 7.2, 0.025 M KCl, 0.005 M MgCl2

TKM:

0.05 M Tris-HCl, pH 7.5, 0.025 M KCl, 0.005 M MgCl2

STM II:

0.1 M NaCl, 0.01 M Tris-HCl, pH 8, 0.001 M MgCl2

DTT:

dithiothreitol

SDS:

sodium dodecylsulphate

References

  1. Lee, S. Y., Mendecki, J., and Brawerman, G., Proc. Nat. Acad. Sci. U.S.A. 68, 1321 (1971).

    Google Scholar 

  2. Greenberg, J. R. and Perry, R. P., J. Mol. Biol. 72, 91 (1972).

    Google Scholar 

  3. Samarina, O. P., Aitkhozhina, N. A., and Besson, J., Mol. Biol. Reports 1, 193 (1973).

    Google Scholar 

  4. Quinlan, T. J., Billings, P. B., Martin, T. E., Proc. Nat. Acad. Sci. U.S.A. 71, 2632 (1974).

    Google Scholar 

  5. Molnar, J. and Samarina, O. P., Mol. Biol. Reports 2, 1 (1975).

    Google Scholar 

  6. Blobel, G., Proc. Nat. Acad. Sci. U.S.A. 70, 924 (1973).

    Google Scholar 

  7. Kish, V. M. and Pederson, T., J. Mol. Biol. 95, 227 (1975).

    Google Scholar 

  8. Molnár, J. and Samarina, O. P., Acta Biochim. Biophys. Acad. Sci. Hung. in press.

  9. Blobel, G. and Potter, R., J. Mol. Biol. 26, 279 (1967).

    Google Scholar 

  10. Shoeness, D., Puckett, L., and Darnell, J. E., Proc. Nat. Acad. Sci. U.S.A. 72, 1077 (1975).

    Google Scholar 

  11. Komáromy, L., Molnár, J., and Tigyi, A., Acta Biochim. Biophys. Acad. Sci. Hung. 10, 207 (1975).

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biology, University Medical School, Pécs, Hungary

    T. Tomcsanyi, J. Molnar & A. Tigyi

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  1. T. Tomcsanyi
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  2. J. Molnar
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  3. A. Tigyi
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Tomcsanyi, T., Molnar, J. & Tigyi, A. Nuclear and polyribosomal ribonucleoprotein particles containing poly(A) in rat liver cells. Mol Biol Rep 3, 99–104 (1976). https://doi.org/10.1007/BF00423222

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  • DOI: https://doi.org/10.1007/BF00423222

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