Archives of Microbiology

, Volume 120, Issue 3, pp 301–302 | Cite as

Interfamilial transfer of amber suppressor gene for the isolation of amber mutants of mycobacteriophage I3

  • T. Ramakrishnan
  • M. S. Shaila
Short Communications


A suppressor-containing strain of Mycobacterium smegmatis SN2 was isolated by transferring an amber suppressor carried on the plasmid of Pseudomonas pseudoalcaligenes ERA through transformation. Amber mutants of mycobacteriophage I3 were isolated.

Key words

Mycobacterium smegmatis Pseudomonas pseudoalcaligenes Plasmid DNA Suppressor Transformation Mycobacteriophage I3 Amber mutants 

Non-Common Abbreviations




Multiplicity of infection


  1. Cohen, S. N., Chang, A. C. Y., Hsu, L.: Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. Nat. Acad. Sci. U.S.A. 69, 2110–2114 (1972)Google Scholar
  2. Elseviers, D., Gorini, L.: Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B. Mol. Gen. Genet. 137, 277–287 (1975)Google Scholar
  3. Gopinathan, K. P., Saroja, D., Gadagkar, R., Ramakrishnan, T.: Control of lysogeny in mycobacteria. In: Genetics of actinomycetales (R. Freerksen, E. Tárnok, H. Thumim, eds.), pp. 237–242. Stuttgart-New York: Fischer 1978Google Scholar
  4. Grange, J. M.: The genetics of mycobacteria and mycobacteriophage — a review. Tubercle 56, 227–232 (1975)Google Scholar
  5. Guerry, P., LeBlanc, D. J., Falkow, S.: General method for the isolation of plasmid Deoxyribonucleic acid. J. Bacteriol. 116, 1064–1066 (1973)Google Scholar
  6. Mindich, L., Cohen, J., Weisburd, M.: Isolation of nonsense suppressor mutants in Pseudomonas. J. Bacteriol. 126, 177–182 (1976)Google Scholar
  7. Söll, L., Berg, P.: Recessive lethals: A new class of nonsense suppressors in Escherichia coli. Proc. Nat. Acad. Sci. U.S.A. 63, 392–399 (1969)Google Scholar
  8. Studier, F. W.: The genetics and physiology of bacteriophage T7. Virology 39, 562–574 (1969)Google Scholar
  9. Sundar Raj, C. V., Ramakrishnan, T.: Transduction in Mycobacterium smegmatis. Nature 228, 280–281 (1970)Google Scholar
  10. Tokunaga, T., Sellers, M. I.: Transfection of amino acid-sensitized mycobacteria. In: “Host-virus relationships in Mycobacterium, Nocardia and Actinomyces” (S. E. Juhasz, G. Plummer, eds.), pp. 152–165. Springfield, Ill.: C. C. Thomas 1970Google Scholar
  11. Youmans, G. P., Karlson, A. G.: Streptomycin sensitivity of tubercle bacilli: studies on recently isolated tubercle bacilli and the development of resistance to streptomycin in vivo. Am. Rev. Tuberc. Pulm. Dis. 55, 529–534 (1947)Google Scholar
  12. Zinder, M. D., Cooper, S.: Host-dependent mutants of the bacteriophage f2. 1. Isolation and preliminary classification. Virology 23, 152–158 (1964)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • T. Ramakrishnan
    • 1
  • M. S. Shaila
    • 1
  1. 1.Microbiology and Cell Biology LaboratoryIndian Institute of ScienceBangaloreIndia

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