Archives of Microbiology

, Volume 120, Issue 3, pp 301–302 | Cite as

Interfamilial transfer of amber suppressor gene for the isolation of amber mutants of mycobacteriophage I3

  • T. Ramakrishnan
  • M. S. Shaila
Short Communications

Abstract

A suppressor-containing strain of Mycobacterium smegmatis SN2 was isolated by transferring an amber suppressor carried on the plasmid of Pseudomonas pseudoalcaligenes ERA through transformation. Amber mutants of mycobacteriophage I3 were isolated.

Key words

Mycobacterium smegmatis Pseudomonas pseudoalcaligenes Plasmid DNA Suppressor Transformation Mycobacteriophage I3 Amber mutants 

Non-Common Abbreviations

NTG

N-methyl-N′-nitro-N-nitrosoguanidine

moi

Multiplicity of infection

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • T. Ramakrishnan
    • 1
  • M. S. Shaila
    • 1
  1. 1.Microbiology and Cell Biology LaboratoryIndian Institute of ScienceBangaloreIndia

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