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Intraspecies aggression in rats: Effects of d-amphetamine and chlordiazepoxide

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  • Animal Studies
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Abstract

d-Amphetamine sulfate and chlordiazepoxide hydrochloride, administered to either the dominant or subordinate rat, altered several components of fighting behavior in a dose-dependent biphasic manner. Stereotypic sequences of attack, threat, defense, and submission were generated between pairs of previously isolated Sprague-Dawley rats by extinction of a food-reinforced response. Low doses of amphetamine (0.05, 0.1 mg/kg) and chlordiazepoxide (2.5, 5.0 mg/kg) given i.m. to the dominant rat 30 min prior to 15 min tests increased attack bites and leaps and the display of aggressive postures and threats, whereas higher doses of both drugs (0.5, 1.0 mg/kg amphetamine; 20 mg/kg chlordiazepoxide) suppressed attacks and threats. Amphetamine and chlordiazepoxide, administered to the subordinate rat, caused a more prolonged display of submissive-supine and defensive-upright postures; chlordiazepoxide (10.0, 20.0 mg/kg) prolonged immobile crouching whereas amphetamine (0.5, 1.0 mg/kg) greatly reduced this response. Drugged subordinate rats also provoked more attacks and threats by the non-drugged opponents. The multi-response analysis of fighting reveals that various elements of aggressive and defensive-submissive behavior patterns are differentially sensitive to drug action. The results indicate that amphetamine and chlordiazepoxide can facilitate or inhibit attack or defense depending on the dose level and which of the opponents was injected, but do not reverse dominance-subordination relationships.

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References

  • Barnett, S. A.: A study in behaviour. Principles of ethology and behavioural physiology, displayed mainly in the rat. London: The Camelot Press 1963

    Google Scholar 

  • Barnett, S. A.: An analysis of social behaviour in wild rats. Proc. Zool. Soc. London 130, 107–152 (1958)

    Google Scholar 

  • Borgesova, M., Kadlecova, O., Krsiak, M.: Behaviour of untreated mice to alcohol- or chlordiazepoxide-treated partners. Activ. nerv. sup. (Praha) 13, 206–207 (1971)

    Google Scholar 

  • Cairns, R. B., Scholz, S. D.: Fighting in mice: dyadic escalation and what is learned. J. comp. physiol. Psychol. 85, 540–550 (1973)

    Google Scholar 

  • Chance, M. R. A.: Ethology and psychopharmacology. In: Psychopharmacology. Dimensions and perspectives. C. R. B. Joyce, ed., pp. 283–318. London: Tavistock 1968

    Google Scholar 

  • Chance, M. R. A., Silverman, A. P.: The structure of social behaviour and drug action. In: Animal behaviour and drug action. H. Steinberg, A. V. S. deReuck, and J. Knight, eds., pp. 65–79. Boston: Little Brown & Co. 1964

    Google Scholar 

  • Christmas, A. J., Maxwell, D. R.: A comparison of the effects of some benzodiazepines and other drugs on aggressive and exploratory behaviour in mice and rats. Neuropharmacology 9, 17–29 (1970)

    Google Scholar 

  • Cole, H. F., Wolf, H. H.: The effects of some psychotropic drugs on conditioned avoidance and aggressive behaviors. Psychopharmacologia (Berl.) 8, 389–396 (1966)

    Google Scholar 

  • Cole, H. F., Wolf, H. H.: Laboratory evaluation of aggressive behavior of the grasshopper mouse (onychomys). J. pharm. Sci. 59, 969–971 (1970)

    Google Scholar 

  • Crowley, T. J.: Dose-dependent facilitation or suppression of rat fighting by methamphetamine, phenobarbital, or imipramine. Psychopharmacologia (Berl.) 27, 213–222 (1972)

    Google Scholar 

  • DaVanzo, J. P., Daugherty, M., Ruckart, R., Kang, L.: Pharmacological and biochemical studies in isolation-induced fighting mice. Psychopharmacologia (Berl.) 9, 210–219 (1966)

    Google Scholar 

  • DiMascio, A.: The effects of benzodiazepines on aggression: reduced or increased? Psychopharmacologia (Berl.) 30, 95–102 (1973)

    Google Scholar 

  • Emley, G. S., Hutchinson, R. R.: Basis of behavioral influence on chlorpromazine, Life Sci. 11, 43–47 (1972)

    Google Scholar 

  • Figler, M. H.: The effects of chlordiazepoxide (Librium) on the intensity and habituation of agonistic behavior in male Siamese fighting fish. Psychopharmacologia (Berl.) 33, 277–292 (1973)

    Google Scholar 

  • Fox, K. A., Snyder, R. L.: Effect of sustained low doses of diazepam on aggression and mortality in grouped male mice. J. comp. physiol. Psychol. 69, 663–666 (1969)

    Google Scholar 

  • Fox, K. A., Tuckosh, J. R., Wilcox, A. H.: Increased aggression among grouped male mice fed chlordiazepoxide. Europ. J. Pharmacol. 11, 119–121 (1970)

    Google Scholar 

  • Fox, K. A., Webster, J. C., Guerriero, F. J.: Increased aggression among grouped male mice fed nitrazepam and flurazepam. Pharmacol. Res. Commun. 4, 157–162 (1972)

    Google Scholar 

  • Grant, E. C., Mackintosh, J. H.: A comparison of the social postures of some common laboratory rodents. Behaviour 21, 246–259 (1963)

    Google Scholar 

  • Grossman, S. P., Sclafani, A.: Sympathomimetic amines. In: Pharmacological and biophysical agents and behavior. E. Furchgott, ed., pp. 269–344. New York: Academic Press 1971

    Google Scholar 

  • Guaitani, A., Marcucci, F., Garattini, S.: Increased aggression and toxicity in grouped male mice treated with tranquilizing benzodiazepines. Psychopharmacologia (Berl.) 19, 241–245 (1971)

    Google Scholar 

  • Heise, G. A., Boff, E.: Taming action of chlordiazepoxide. Fed. Proc. 20, 393 (1961)

    Google Scholar 

  • Hoffmeister, F., Wuttke, W.: On the actions of psychotropic drugs on the attack and aggressive-defensive behaviour of mice and cats. In: Aggressive behaviour. S. Garattini and E. B. Sigg, eds., pp. 273–280. Amsterdam: Excerpta Medica Foundation 1969

    Google Scholar 

  • Horovitz, Z. P., Ragozzino, P. W., Leaf, R. C.: Selective block of rat mouse-killing by antidepressants. Life Sci. 4, 1909–1912 (1965)

    Google Scholar 

  • Karli, P.: Action de l'amphétamine et de la chlorpromazine sur l'aggressivité interspecifique Rat-Souris. C. R. Soc. Biol. (Paris) 152, 1796–1798 (1958)

    Google Scholar 

  • Karli, P.: Richerches pharmacologiques sur le comportement d'aggressivité Rat-Souris. J. Physiol. (Paris) 51, 497–498 (1959)

    Google Scholar 

  • Krsiak, M., Borgesova, M.: Effect of alcohol on behaviour of pairs of rats. Psychopharmacologia (Berl.) 32, 201–209 (1973)

    Google Scholar 

  • Krsiak, M., Steinberg, H.: Psychopharmacological aspects of aggression: a review of the literature and some new experiments. J. psychosom. Res. 13, 243–252 (1969)

    Google Scholar 

  • Lal, H., Defeo, J. J., Thut, P.: Effect of amphetamine on pain-induced aggression. Commun. Behav. Biol., Part A 1, 333–336 (1968)

    Google Scholar 

  • Langfeldt, T., Ursin, H.: Differential action of diazepam on flight and defense behavior in the cat. Psychopharmacologia (Berl.) 19, 61–66 (1971)

    Google Scholar 

  • Miczek, K. A., Barry, H.: δ 9-tetrahydrocannabinol and aggressive behavior in rats. Behav. Biol. 11, 261–267 (1974)

    Google Scholar 

  • Miczek, K. A., Brykczynski, T., Grossman, S. P.: Differential effects of lesions in the amygdala, periamygdaloid cortex, or stria terminalis on aggressive behaviors in rats. J. comp. Physiol. Psychol. (in press) (1974)

  • Miczek, K. A., Grossman, S. P.: Effects of septal lesions on inter- and intraspecies aggression in rats. J. comp. physiol. Psychol. 79, 37–45 (1972)

    Google Scholar 

  • Milner, G.: Modified confinement motor activity test for use in mice. J. pharm. Sci. 57, 1900–1902 (1968)

    Google Scholar 

  • Norton, S.: Amphetamine as a model for hyperactivity in the rat. Physiol. Behav. 11, 181–186 (1973)

    Google Scholar 

  • Panksepp, J.: Drugs and stimulus-bound attack. Physiol. Behav. 6, 317–320 (1971)

    Google Scholar 

  • Quenzer, L. F., Feldman, R. S., Moore, J. W.: Toward a mechanism of the antiaggression effects of chlordiazepoxide in rats. Psychopharmacologia (Berl.) 34, 81–94 (1974)

    Google Scholar 

  • Randall, L. O.: Pharmacology of methaminodiazepoxide. Dis. nerv. Syst. 21, 7–15 (1960)

    Google Scholar 

  • Randall, L. O., Schallek, W.: Pharmacological activity of certain benzodiazepines. In: Psychopharmacology. A review of progress. D. H. Efron, ed., pp. 153–184. Washington, D. C.: U.S. Government Printing Office, 1968

    Google Scholar 

  • Randall, L. O., Schallek, W., Heise, G. A., Keith, E. F., Bagdon, R. E.: The psychosedative properties of methaminodiazepoxide. J. Pharmacol. exp. Ther. 129, 163–171 (1960)

    Google Scholar 

  • Randrup, A., Munkvad, I.: Relation of brain catecholamines to aggressiveness and other forms of behavioural excitation. In: Aggressive behaviour. S. Garattini and E. B. Sigg, eds., pp. 228–235. Amsterdam: Excerpta Medica Foundation 1969

    Google Scholar 

  • Richardson, D., Karczmar, A. G., Scudder, C. L.: Intergeneric behavioral differences among methamphetamine treated mice. Psychopharmacologia (Berl.) 25, 347–375 (1972)

    Google Scholar 

  • Scheckel, C. L., Boff, E.: Effects of drugs on aggressive behavior in monkeys. Excerpta Medica International Congress Series 129, 789–795 (1966)

    Google Scholar 

  • Sheard, M. H.: The effects of amphetamine on attack behavior in the cat. Brain Res. 5, 330–338 (1967)

    Google Scholar 

  • Siegel, S.: Nonparametric statistics for the behavioral sciences. New York: McGraw Hill 1956

    Google Scholar 

  • Silverman, A. P.: Behaviour of rats given a “smoking dose” of nicotine. Anim. Behav. 19, 67–74 (1971)

    Google Scholar 

  • Silverman, A. P.: Ethological and statistical analysis of drug effects on the social behaviour of laboratory rats. Brit. J. Pharmacol. 24, 579–590 (1965)

    Google Scholar 

  • Sofia, R. D.: Structural relationship and potency of agents which selectively block mouse killing (muricide) behavior in rats. Life Sci. 8, 1201–1210 (1969)

    Google Scholar 

  • Spigel, I. M., Trivett, S., Fraser, D.: Grooming behavior and competitive dominance in the albino rat. J. comp. physiol. Psychol. 78, 409–411 (1972)

    Google Scholar 

  • Stark, P., Henderson, J. K.: Differentiation of classes of neurosedatives using rats with septal lesions. Int. J. Neuropharmacolog. 5, 385–389 (1966)

    Google Scholar 

  • Tedeschi, D. H., Fowler, P. J., Cromley, W. H., Paulis, J. F., Eby, R. Z., Fellows, E. J.: Effects of centrally acting drugs on confinement of motor activity. J. pharm. Sci. 53, 1046–1058 (1964)

    Google Scholar 

  • Valzelli, L.: Activity of benzodiazepines on aggressive behavior in rats and mice. In: The benzodiazepines. S. Garattini, E. Mussini, and L. O. Randall eds., pp. 405–417. New York: Raven Press 1973

    Google Scholar 

  • Valzelli, L.: Drugs and aggressiveness. Advanc. Pharmacol. 5, 79–108 (1967)

    Google Scholar 

  • Valzelli, L., Giacalone, E., Garattini, S.: Pharmacological control of aggressive behavior in mice. Europ. J. Pharmacol. 2, 144–146 (1967)

    Google Scholar 

  • Welch, B. L., Welch, A. S.: Aggression and the biogenic amine neurohumors. In: Aggressive behaviour. S. Garattini and E. B. Sigg, eds., pp. 188–202. Amsterdam. Excerpta Medica Foundation 1969

    Google Scholar 

Download references

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  1. Department of Psychology, Carnegie-Mellon University, Pittsburgh, Pennsylvania

    Klaus A. Miczek

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Miczek, K.A. Intraspecies aggression in rats: Effects of d-amphetamine and chlordiazepoxide. Psychopharmacologia 39, 275–301 (1974). https://doi.org/10.1007/BF00422968

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  • DOI: https://doi.org/10.1007/BF00422968

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