Molecular and General Genetics MGG

, Volume 186, Issue 1, pp 106–110 | Cite as

Reiterated DNA sequences in a mutant strain of Streptomyces glaucescens and cloning of the sequence in Escherichia coli

  • Hideo Ono
  • Gilberto Hintermann
  • Reto Crameri
  • Gillian Wallis
  • Ralf Hütter
Article

Summary

Streptomyces glaucescens exhibits a high degree of genetic instability. A sequence of 7.2 kb has been found which is present in a few tandemly repeated copies in the wild type strain GLA 0 and is amplified to ca. 500 copies per genome in the mutant strain GLA 1204. This sequence was cloned in Escherichia coli using pBR325 as vector.

Keywords

Escherichia Coli Streptomyces Type Strain Mutant Strain Wild Type Strain 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Hideo Ono
    • 1
  • Gilberto Hintermann
    • 1
  • Reto Crameri
    • 1
  • Gillian Wallis
    • 1
  • Ralf Hütter
    • 1
  1. 1.Institute of MicrobiologySwiss Federal Institute of TechnologyZürichSwitzerland

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