Advertisement

Molecular and General Genetics MGG

, Volume 186, Issue 1, pp 57–65 | Cite as

Regulation of DNA synthesis and capacity for initiation in DNA temperature sensitive mutants of Escherichia coli

I. Reinitiation and chain elongation
  • Helen Eberle
  • Nancy Forrest
  • Juri Hrynyszyn
  • Janice Van Knapp
Article

Summary

The capacity for initiation and subsequent chain elongation was examined in several DNA temperature sensitive mutants of Escherichia coli after the mutants had been held at nonpermissive temperature for approximately 1.5 generation equivalents and then returned to permissive temperature in the presence of chloramphenicol. The results obtained indicate that 4–5 sets of replication forks can be initiated after return to permissive temperature in the presence of chloramphenicol but the forks apparently become stalled and fail to complete chromosomal replication in the presence of chloramphenicol. In temperature reversible dnaA mutants, once the chloramphenicol is removed the forks appear to be able to resume replication at the nonpermissive temperature. The relationship between premature initiation and premature chain termination is discussed.

Keywords

Escherichia Coli Chloramphenicol Replication Fork Chain Termination dnaA Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abe M, Tomizawa J (1971) Chromosome replication in a Escherichia coli K-12 mutant affected in the process of DNA initiation. Genetics 69:1–15Google Scholar
  2. Bachmann BJ, Low KB, Taylor AL (1976) Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev 40:116–167Google Scholar
  3. Beyersmann D, Schlicht M, Schuster H (1971) Temperature-sensitive initiation of DNA replication in a mutant of Escherichia coli K-12. Mol Gen Genet 111:145–158Google Scholar
  4. Bird R, Lark KG (1968) Initiation and termination of DNA replication after amino acid starvation of E. coli 15T. Cold Spring Harbor Symp Quant Biol 33:799–808Google Scholar
  5. Bird RE, Louran J, Martuscelli J, Caro LG (1972) Origin and sequence of chromosome replication in Escherichia coli. J Mol Biol 70:549–556Google Scholar
  6. Burton K (1956) A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323Google Scholar
  7. Carl PL (1970) Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet 109:107–122Google Scholar
  8. Clark L, Carbon J (1976) A colony back containing synthetic Col El hybrid plasmids representative of the entire Escherichia coli genome. Cell 9:91–99Google Scholar
  9. Cohen SN, Miller CA (1970) Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli. J Mol Biol 50:671–687Google Scholar
  10. Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646Google Scholar
  11. Evans I, Eberle H (1975) Accumulation of the capacity for initiation of deoxyribonucleic acid replication in Escherichia coli. J Bacteriol 121:883–891Google Scholar
  12. Evans IM, Forrest N, Lawrence A, Eberle H (1979) Effect of blocking protein synthesis at nonpermissive temperatures on temperaturesensitive deoxyribonucleic acid mutants of Escherichia coli. J Bacteriol 140:445–451Google Scholar
  13. Fralick JA, Lark KG (1973) Evidence for the involvement of unsaturated fatty acids in initiating chromosome replication in Escherichia coli. J Mol Biol 80:459–475Google Scholar
  14. Hansen FG, Rasmussen KV (1977) Regulation of the dnaA product in Escherichia coli. Mol Gen Genet 155:219–225Google Scholar
  15. Hayward GS, Smith MG (1972) The chromosome of bacteriophage T5. I. Analysis of the single stranded DNA fragments by agarose gel electrophoresis. J Mol Biol 63:383–396Google Scholar
  16. Helmstetter CE, Cooper S, Pierucci O, Revelas E (1968) On the bacterial life sequence. Cold Spring Harbor Symp Quant Biol 33:804–822Google Scholar
  17. Helmstetter CE, Pierucci O (1968) Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol 95:1627–1633Google Scholar
  18. Hiraga S, Saitoh T (1974) Initiation of DNA replication in Escherichia coli. I. Characteristics of the initiation process in dna mutants. Mol Gen Genet 132:49–62Google Scholar
  19. Hirota Y, Mordoh J, Jacob F (1970) On the process of cellular division in Escherichia coli. III. Thermosensitive mutants of E. coli altered in the process of DNA initiation. J Mol Biol 53:369–388Google Scholar
  20. Kellenberger-Gujer G, Podhajska AJ, Caro L (1978) A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperatures. Mol Gen Genet 162:9–16Google Scholar
  21. Lark KG, Repko T, Hoffman EJ (1963) The effect of amino acid deprivation on subsequent DNA replication Biochim Biophys Acta 76:9–24Google Scholar
  22. Lark KG (1973) Initiation and termination of bacterial deoxyribonucleic acid replication in low concentrations of chloramphenicol. J Bacteriol 113:1066–1069Google Scholar
  23. Lycett GW, Orr E, Pritchard RH (1980) Chloramphenicol releases a block in initiation of chromosome replication in a draA strain of Escherichia coli K12. Mol Gen Genet 178:329–336Google Scholar
  24. Maaløe O, Hanawalt PC (1961) Thymine deficiency and the normal DNA replication cycle. J Mol Biol 3:114–155Google Scholar
  25. Marsh RC, Worcel A (1977) A DNA fragment containing the origin of replication of the Escherichia coli chromosome. Proc Natl Acad Sci USA 74:2720–2724Google Scholar
  26. Messer W (1972) Initiation of deoxyribonucleic acid replication of Escherichia coli B/r: Chronology of events and transcriptional control of initiation. J Bacteriol 112:7–12Google Scholar
  27. Messer W, Dankwarth L, Tippe-Schindler R, Womack J, Zahn G (1975) Regulation of the initiation of DNA replication in Escherichia coli. Isolation of I-RNA and the control of I-RNA synthesis. In: Goulian M, Hanawalt P, Fox CF (eds). Proc ICN-UCLA Symp Mol and Cell Biol, vol III, DNA synthesis and its regulation. WA Benjamin, Inc, Menlo Park. California, pp 602–617Google Scholar
  28. Orr E, Meacock PA, Pritchard RH (1978) Genetic and physiological properties of an E. coli strain carrying the dnaA mutation T46. In: Molineux I, Kohiyama M (eds). DNA synthesis: Present and future. Plenum Publishing Corp, New York and London, pp 85–99Google Scholar
  29. Pato M (1975) Alterations of the rate of movement of deoxyribonucleic acid replication forks. J Bacteriol 123:272–277Google Scholar
  30. Pritchard RH, Lark KG (1965) Induction of replication by thymine starvation at the chromosome origin in Escherichia coli. J Mol Biol 9:288–307Google Scholar
  31. Pritchard RH, Zaritsky A (1970) Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli. Nature (Lond) 226:126–131Google Scholar
  32. Rodriguez RL, Davern CI (1976) Direction of deoxyribonucleic acid replication in Escherichia coli under various conditions of cell growth. J Bacteriol 125:346–352Google Scholar
  33. Schwartz M, Worcel A (1971) Reinitiation of chromosome replication in a thermosensitive DNA mutant of Escherichia coli. II. Synchronization of chromosome replication after temperature shifts. J Mol Biol 61:329–342Google Scholar
  34. Stein G, Hanawalt P (1969) Initiation of DNA replication cycles in Escherichia coli following DNA synthesis inhibition. J Mol Biol 46:135–444Google Scholar
  35. Sueoka N, Yoshikawa H (1965) The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics 52:747–757Google Scholar
  36. van Meyenburg K, Hansen FG, Nielsen LD, Jorgensen P (1977) Origin of replication, oriC, of the Escherichia coli chromosome: mapping of genes relative to the EcoRI cleavage sites in oriC region. Mol Gen Genet 158:101–109Google Scholar
  37. Ward CB, Glaser DA (1970) Control of initiation of DNA synthesis in Escherichia coli B/r. Proc Natl Acad Sci USA 67:255–262Google Scholar
  38. Wechsler JA, Gross JD (1971) Escherichia coli mutants temperaturesensitive for DNA synthesis. Mol Gen Genet 113:273–284Google Scholar
  39. Wickner SH (1978) DNA replication proteins of Escherichia coli. Annu Rev Biochem 47:1163–1191Google Scholar
  40. Worcel A (1970) Induction of chromosome re-initiations in a thermosensitive DNA mutant of Escherichia coli. J Mol Biol 52:371–386Google Scholar
  41. Yasuda S, Hirota Y (1977) Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci USA 74:5458–562Google Scholar
  42. Zahn G, Messer W (1979) Control of the initiation of DNA replication in Escherichia coli. II. Function of the dnaA product. Mol Gen Genet 168:197–209Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Helen Eberle
    • 1
  • Nancy Forrest
    • 1
  • Juri Hrynyszyn
    • 1
  • Janice Van Knapp
    • 1
  1. 1.Department of Radiation Biology and BiophysicsUniversity of Rochester, School of Medicine and DentistryRochesterUSA

Personalised recommendations