Summary
The effect of 5-day sleep deprivation (SD) on cholesterol metabolism, together with triglyceridaemia, was studied in seven healthy male volunteers. A 3-day control period was followed by 5 days (120 h) complete SD and 4 days recovery. Blood was collected at 9 a.m. and at 9 p.m. Vastus lateralis muscle biopsy was performed during the control period, on the 5th day of SD, and on day 3 of recovery. The value of muscle cholesterol was related to the non-collagen protein content. The plasma triglycerides (TG) varied in a circadian biorhythm, the amplitude of which declined gradually during SD. The morning triglyceridaemia was significantly decreased on days 3–5 of SD (35%–79% of initial values). On days 4 and 5 of SD, plasma cholesterol fell significantly to 78% and 88% of control values, respectively. The ratio of its esterified to unesterified fractions remained unchanged throughout SD. Basal activity of lecithin cholesterol acyltransferase (LCAT) showed no diurnal biorhythm; on the last 2 days of SD, LCAT activity fell significantly to 71%–80%. In contrast, the decrease in fractional esterification rate (FER) was insignificant. In the vastus lateralis muscle, total cholesterol (TC) was decreased by 40% at the end of SD, the reduction being greater for cholesterol esters (CE) (by 63%) than for free cholesterol (FC) (by 36%). The relative proportion of CE significantly decreased from an initial 14.7% to 9.2% on the last day of SD. During recovery after SD, plasma cholesterol and TG slowly returned to normal. LCAT activity and FER recovered quickly, within 48 h. In the muscle, recovery was characterized by cholesterol accumulation, particularly by that of its esterified fraction: TC increased by 11%, CE by 57%, and FC by 3% over the respective control values. The percentage of CE rose to 20.7%. It may be concluded that SD has a profound influence on cholesterol metabolism. Some changes resemble those characterizing cardiovascular diseases.
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Berlin T, Cronestrand R, Nowak T, Sonnenfeld T, Wennmalm å (1979) Conversion of arachidonic acid to prostaglandins in homogenates of human skeletal muscle and kidney. Acta Physiol Scand 106:441–445
Blomhoff JP (1973) Serum cholesterol determination by gasliquid chromatography. Clin Chim Acta 43:257–265
Cooper RA, Shattil SJ (1980) Membrane cholesterol — is enough too much? N Engl J Med 302:49–50
Dobiášová M, Vondra K (1978) Lecithin: cholesterol acyltransferase as a possible diagnostic tool in ischemic heart disease. Scand J Clin Lab Invest [Suppl] 150:129–133
Dobiášová M, Kopecká J, Živný K (1978) Lecithin: cholesterol acyltransferase determination. čas Lék česk 117:1394–1396
Franey JR, Amádor E (1968) Serum cholesterol measurement based on ethanol extraction and ferric chloride-sulphuric acid. Clin Chim Acta 21:255–263
Glomset JA, Wright JL (1964) Some properties of a cholesterol-esterifying enzyme in human plasma. Biochim Biophys Acta 89:266–276
Grafnetter D (1973) A simplified estimation of triglycerides for diagnosis of hyperlipoproteinaemia. VnitŘní Lék 19:808–815
Henry RJ, Cannon DC, Winkelman JW (1974) Clinical chemistry, principles, and technics, 2nd edn. Harper and Row, Hagerstown, Nd., p 1629
Lilienthal JL Jr, Zierler KL, Folk BP, Buka R, Riley MJ (1950) A reference case and system for analyses of muscle constituents. J Biol Chem 182:501–508
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Marcel Y, Vezina X (1973) A method for the determination of the initial rate of reaction of lecithin: cholesterol acyltransferase in human plasma. Biochim Biophys Acta 306:497–504
Nicolaysen R, Nygaard AP (1963) The determination of triglycerides and phospholipids. Scand J Clin Lab Invest 15:79–82
Sinclair HM (1980) Prevention of coronary heart disease: the role of essential fatty acids. Postgrad Med J 56:579–584
Small DM (1977a) Cellular mechanisms for lipid deposition in atherosclerosis. I. N Engl J Med 297:873–877
Small DM (1977b) Cellular mechanisms for lipid deposition in atherosclerosis. II. N Engl J Med 297:924–929
Vondra K, Rath R, Kroupa Z (1974) Improved needle for muscle biopsy. Klin Wochenschr 52:747–748
Vondra K, Brodan V, Bass A, Kuhn E, AndĚl M, Vítek V, Teisinger J (1982) The effect of short maximum load exercise test on some enzyme activities and cholesterol content in human skeletal muscle. In: Metabolic and functional changes during exercise. Proceedings of the Satellite Symposium of the XXVIIIth International Congress of Physiological Sciences, Prague, pp 53–56
Vondra K, Dobiášová M, Vítek V, Bass A, Válek J, Kopecká J, Grafnetter D (1985) Fractional cholesterol esterification rate and muscle cholesterol of healthy young men. Physiol Bohemoslov 34:313–319
Wolinsky H, Fowler E (1978) Participation of lysosomes in atherosclerosis. Seminars in medicine of the Beth Israel Hospital, Boston. N Engl J Med 299:1173–1178
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Vondra, K., Brodan, V., Dobiášová, M. et al. Effect of sleep deprivation on cholesterol metabolism and triglyceridaemia in male volunteers. Europ. J. Appl. Physiol. 55, 83–87 (1986). https://doi.org/10.1007/BF00422899
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DOI: https://doi.org/10.1007/BF00422899