Skip to main content

Advertisement

SpringerLink
Log in

Unusual cytochemical reactivity in canine acute myeloblastic leukaemia

  • Case Report
  • Published:
Comparative Haematology International Aims and scope Submit manuscript

Abstract

A case of acute myeloblastic leukaemia with unusual cytochemical reactivity in a dog is reported. Blood and bone marrow examination revealed predominantly highly undifferentiated leucocytes lacking peroxidase, sudan black B, and alpha-naphthyl acetate esterase activities. Most of the immature cells in blood and bone marrow smears showed strong alkaline phosphatase activity. Transmission electron microscopy of bone marrow cells revealed features of myeloid differentiation particularly secondary granules. These findings supported the diagnosis of acute myeloblastic leukaemia without differentiation (Ml) under the FAB system of classification of acute myeloid leukaemas in humans.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abe M, Nozawa Y, Wachi E, Tominaga K, Hojo H, Wakasa H (1988) Alkaline phosphatase-positive B cell lymphomas. Eur J Haematol 41:223–229

    Google Scholar 

  • Bainton DF, Farquhar MG (1968) Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes II. Cytochemistry and electron microscopy of bone marrow cells. J Cell Biol 39:299–317

    Google Scholar 

  • Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (1985) Proposed revised criteria for the classification of acute myeloid leukemia. Ann Intern Med 103:620–625

    Google Scholar 

  • Facklam NR, Kociba GJ (1985) Cytochemical characterization of leukemic cells from 20 dogs. Vet Pathol 22:363–369

    Google Scholar 

  • Grindem CB, Stevens JB, Perman V (1985) Morphologic classification and clinical and pathologic characteristics of spontaneous leukemia in 17 dogs. J Am Anim Hosp Assoc 21:219–227

    Google Scholar 

  • Grindem CB, Stevens JB, Perman V (1986) Cytochemical reactions in cells from leukemic dogs. Vet Pathol 23:103–109

    Google Scholar 

  • Jain NC (1986) Schalm's veterinary hematology, Lea & Febiger. Philadelphia, Chapter 33, pp 909–939

    Google Scholar 

  • Jain NC, Madewell BR, Weller RE, Geissler MC (1981) Clinicopathological findings and cytochemical characterization of myelomonocytic leukemia in 5 dogs. J Comp Pathol 91:17–31

    Google Scholar 

  • Lee EJ, Pollak A, Leavitt RD, Testa JR, Schiffer CA (1987) Minimally differentiated acute nonlymphocytic leukemia: a distinct entity. Blood 70: 1400–1406

    Google Scholar 

  • Nanba K, Jaffe ES, Braylan RC, Soban EJ, Berard CW (1977) Alkaline phosphatase-positive malignant lymphoma. a subtype of B-cell lymphomas. Am J Clin Pathol 68:535–542

    Google Scholar 

  • Neumann H, Klein E, Hauck-Granoth R, Yachnin S, Ben-Bassat H (1976) Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells. Pro Nat Acad Sci 73:1432–1436

    Google Scholar 

  • Palakavongs P, Teichberg S, Vinciguerra V, Degnan TJ, Sinlaratana P (1977) Ultrastructural cytochemical analysis of blastic transformation of chronic myelocytic leukemia. Blood49:535–547

    Google Scholar 

  • Polli N, Lambertenghi- Deliliers G, Schiro R, Cattoretti G, Soligo D, Cantu-Rajnoldi A, Romitti L, Delia D, Polli EE (1989) Relevance of ultrastructural immunocytochemistry in the characterization of unclassifiable leukemias: correlation with phenotypic and genic studies. Haematologica (Pavia) 74:129–136

    Google Scholar 

  • Raskin RE, Noriega M (1990) Cytochemical staining of lymph nodes from normal and lymphoma-affected dogs. Veterinary Cancer Society, 10th Annual Conference 21 (Abstract)

  • Schwarze E-W (1980) Cytochemical methods. In: van den Tweel JG et al. (eds.) Malignant lymphoproliferative diseases. Martinus Nijhoff, Boston, p 137

    Google Scholar 

  • Sonoda M, Kobayashi K (1970) Neutrophils of canine peripheral blood in electron microscopy. Jpn J Vet Res 18:37–41

    Google Scholar 

  • van Wering ER, Brederoo P, van Dijk-de Leeuw JHS, van der Meulen J, van't Veer MB (1990) Electron microscopy: a contribution to further classification of acute unclassifiable childhood leukemia. Blut 60:291–296

    Google Scholar 

  • Wellman ML, Couto CG, Starkey RJ, Rojko JL (1989) Lymphocytosis of large granular lymphocytes in three dogs. Vet Pathol 26:158–163

    Google Scholar 

  • Wilson PD, Rustin GJS, Peters TJ (1981) The ultrastructural localization of human neutrophil alkaline phosphatase in normal individuals during pregnancy and in patients with chronic granulocytic leukemia. Histochem J 13:31–43.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Veterinary Surgery, School of Veterinary Medicine, University of California, Davis, California, USA

    B. R. Madewelll

  2. Departments of Clinical Pathology, School of Veterinary Medicine, University of California, Davis, California, USA

    N. C. Jain

  3. Department of Pathology, School of Medicine, University of California, Davis, California, USA

    R. J. Munn

Authors
  1. B. R. Madewelll
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. C. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. J. Munn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Madewelll, B.R., Jain, N.C. & Munn, R.J. Unusual cytochemical reactivity in canine acute myeloblastic leukaemia. Comparative Haematology International 1, 117–120 (1991). https://doi.org/10.1007/BF00422882

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00422882

Keywords

Search

Navigation