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Transfection of protoplasts of Bacillus subtilis with ϕ29 DNA

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Summary

The protoplast-polyethyleneglycol(PEG) transformation procedure of Chang and Cohen (2) can be used for ϕ29 DNA transfection. ϕ29 DNA without terminal proteins is not transfectious in the protoplast-PEG procedure.

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, University of Iowa City, 52242, Iowa, USA

    Donald P. Stahly

  2. Department of Molecular and Medical Microbiology, College of Medicine, University of Arizona, 85724, Tucson, Arizona, USA

    Junetsu Ito

Authors
  1. Donald P. Stahly
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  2. Junetsu Ito
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Communicated by G. O'Donovan

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Stahly, D.P., Ito, J. Transfection of protoplasts of Bacillus subtilis with ϕ29 DNA. Molec. Gen. Genet. 182, 180–182 (1981). https://doi.org/10.1007/BF00422788

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  • DOI: https://doi.org/10.1007/BF00422788

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