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The action of the Notch locus in Drosophila melanogaster

I. Effects of the Notch8 deficiency on mitochondrial enzymes

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Summary

It is shown that the Notch8 deficiency in Drosophila melanogaster affects a number of enzyme activities localized in the mitochondria, such as NADH oxidase (activity of the complete respiratory chain), NADH dehydrogenase (the first step in the respiratory chain before transfer to ubiquinone), Succinate dehydrogenase and α-Glycerophosphate dehydrogenase. The experiments reported here do not exclude the possibility of involvement of other genes in the deficiency. The effect of duplications of the Notch locus on NADH oxidase and NADH dehydrogenase suggest that this locus determines the enzyme activities.

The dosage effects of the Notch locus on activity suggest that this locus contains the structural genes for these enzymes.

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References

  • Ackrell BAC, Kearney EB, Coles CJ, Singer TP, Beinert H, Wan Y, Folkers K (1977) Kinetics of the reoxidation of Succinate dehydrogenase. Arch Biochem Bioph 182:107–117

    Google Scholar 

  • Ackrell BAC, Kearney EB, Mali A, Ludwig B, Capaldi RA (1978) Interactions between Succinate dehydrogenase and its membrane environment. In: Singer T, Ondara RN (eds) Mechanisms of oxidizing enzymes. Developments in Biochemistry, vol 1, 143–154. Elsevier/North Holland Amsterdam New York Oxford

    Google Scholar 

  • Bergh SG van den, Slater EC (1962) The respiratory activity and permeability of Housefly sarcosomes. Biochem J 82:362–371

    Google Scholar 

  • Boell EJ, Poulson DF (1939) The respiratory mechanism of normal and genetically different eggs of Drosophila melanogaster. Anat Record 75:65–66

    Google Scholar 

  • Bos M, Scharloo W, Bijlsma R, De Boer IM, Den Hollander J (1969) Induction of morphological aberrations by enzyme inhibition in Drosophila melanogaster. Experienta 24:811–812

    Google Scholar 

  • Britten RJ, Davidson EH (1969) Gene regulation for higher cells: a theory. Science 165:349–357

    Google Scholar 

  • Coles J, Singer TP, White GA, Thorn GD (1978) Studies on the binding of carboxin analogs to Succinate dehydrogenase. J Biol Chem 253:5573–5578

    Google Scholar 

  • Colowick SP, Kaplan NO (1968) Methods in Enzymology, Vol X. Oxidation and Phosphorylation, second printing, p 3–378. Academic Press, New York and London

    Google Scholar 

  • Cunningham EB (1978) Electron transport and energy production. In: Jackson DC (ed) Biochemistry Mechanisms of metabolism, p 442–473. McGraw Hill, New York

    Google Scholar 

  • Cross DP, Sang JH (1978a) Cell culture of individual Drosophila embryos I. Development of wild-type cultures. J Embryol Exp Morphol 45:161–172

    Google Scholar 

  • Cross DP, Sang JH (1978b) Cell culture of individual Drosophila embryos II. Culture of X-linked embryonic lethals. J Embryol Exp Morphol 45:173–187

    Google Scholar 

  • Demerec M (1934) Biological action of small deficiencies of X-chromosome of Drosophila melanogaster. Proc Natl Acad Sci USA 20:354–359

    Google Scholar 

  • Demerec M (1936) Frequency of “cell-lethals” among lethals obtained at random in the X-chromosome of Drosophila melanogaster. Proc Natl Acad Sci USA 22:350–354

    Google Scholar 

  • Dijken FR van, Scharloo W (1979) Divergent selection on locomotor activity in Drosophila melanogaster I. Selection response. Behav. Genet 9:543–553

    Google Scholar 

  • Foster GG (1973) Temperature-sensitive mutations in Drosophila melanogaster XIII. Temperature-sensitive periods of the lethal and morphological phenotypes of selected combinations of Notch locus mutations. Develop Biol 32:282–296

    Google Scholar 

  • Keppy DO, Welshons WJ (1977) The cytogenetics of a recessive visible mutant associated with a deficiency adjacent to the Notch locus in Drosophila melanogaster. Genetics 85:497–506

    Google Scholar 

  • Li JC (1927) The effect of chromosome aberrations on development in Drosophila melanogaster. Genetics 12:1–58

    Google Scholar 

  • Lindsley DL, Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Institution, Washington, DC

    Google Scholar 

  • Mohr OL (1919) Character changes caused by mutation of an entire region of a chromosome in Drosophila. Genetics 4:275–282

    Google Scholar 

  • Morgan TH, Schultz J, Curry V (1941) Investigations on the constitution of the germinal material in relation to heredity. Carnegie Inst washington, Yearb 40:283

    Google Scholar 

  • Nørby S (1970) A specific nutritional requirement for pyrimidines in rudimentary mutants of Drosophila melanogaster. Hereditas 66:205–214

    Google Scholar 

  • Nørby S (1973) The biochemical genetics of rudimentary mutants of Drosophila melanogaster. Hereditas 73:11–16

    Google Scholar 

  • O'Brien SJ, MacIntyre RJ (1972) The α-Glycerophosphate cycle in Drosophila melanogaster. I. Biochemical and Developmental aspects. Biochemi Genet 7:141–161

    Google Scholar 

  • O'Brien SJ, Gethmann RC (1973) Segmental aneuploidy as a probe for structural genes in Drosophila: Mitochondrial membrane enzymes. Genetics 75:155–167

    Google Scholar 

  • Pearse AGE (1972) Histochemistry. Theoretical and Applied, Vol 2, Third edition, p 891. Churchill Livingstone, Edinburgh and London

    Google Scholar 

  • Peeples EE, Barnett DR, Oliver CP (1968) Phenol oxidases of a lozenge mutant of Drosophila. Science 159:548–552

    Google Scholar 

  • Peeples EE, Geisler A, Whitcraft CJ, Oliver CP (1969a) Comparative studies of phenol oxidase activity during pupal development of three lozenge mutants (lz8, lz, lzk) of Drosophila melanogaster. Genet 62:161–170

    Google Scholar 

  • Peeples EE, Geisler A, Whitcraft CJ, Oliver CP (1969b) Activity of phenol oxidases at the puparium formation stage in development of nineteen lozenge mutants of Drosophila melanogaster. Biochem Genet 3:563–569

    Google Scholar 

  • Portin P (1975) Allelic negative complementation at the Abruptex locus of Drosophila melanogaster. Genet 81:121–133

    Google Scholar 

  • Portin P (1980) On the cell lethality of Notch and Abruptex mutations of Drosophila melanogaster. Hereditas 92:303–307

    Google Scholar 

  • Poulson DF (1939) Effects of Notch deficiencies. Drosophila Inform Serv 12:64–65

    Google Scholar 

  • Poulson DF (1940) The effects of certain X-chromosome deficiencies on the embryonic development of Drosophila melanogaster. J Expt Zool 83:271–326

    Google Scholar 

  • Poulson DF (1945) Chromosomal control of embryogenesis in Drosophila. Am Natural 79:340–363

    Google Scholar 

  • Poulson DF (1968) The embryogenetic function of the Notch locus in Drosophila melanogaster. Proc 12th Intern Congr Genet, Tokyo 1968, 1:143

    Google Scholar 

  • Rawls JM, Fristrom JW (1975) A complex genetic locus that controls the first three steps of pyrimidine biosynethesis in Drosophila. Nature 255:738–740

    Google Scholar 

  • Shellenbarger DL, Mohler JD (1975) Temperature-sensitive mutations of the Notch locus in Drosophila melanogaster. Genet 81:143–162

    Google Scholar 

  • Slizynska H (1938) Salivary chromosome analysis of the white-facet region of Drosophila melanogaster. Genet 23:291–299

    Google Scholar 

  • Thörig GEW, Schoone AA, Scharloo W (1975) Variation between electrophoretically identical alleles at the alcohol dehydrogenase locus in Drosophila melanogaster. Biochemi Genet 13:721–731

    Google Scholar 

  • Welshons WJ, von Halle ES (1962) Pseudoallelism at the Notch locus in Drosophila. Genet 47:743–759

    Google Scholar 

  • Welshons WJ (1965) Analysis of a gene in Drosophila. Science 150:1122–1129

    Google Scholar 

  • Welshons WJ (1971) Genetic basis for two types of recessive lethality at the Notch locus of Drosophila. Genet 68:259–268

    Google Scholar 

  • Welshons WJ (1974) The cytogenetic analysis of a fractured gene in Drosophila. Genet 76:775–794

    Google Scholar 

  • Welshons WJ, Keppy DO (1975) Intragenic deletions and salivary band relationships in Drosophila. Genet 80:143–155

    Google Scholar 

  • Wright ThRF (1970) The genetics of embryogenesis in Drosophila. Advan Genet 15:261–395

    Google Scholar 

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Authors and Affiliations

  1. Department of Population and Evolutionary Biology, Rijksuniversiteit Utrecht, The Netherlands

    G. E. W. Thörig, P. W. H. Heinstra & W. Scharloo

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  1. G. E. W. Thörig
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  2. P. W. H. Heinstra
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  3. W. Scharloo
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Communicated by M.M. Green

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Thörig, G.E.W., Heinstra, P.W.H. & Scharloo, W. The action of the Notch locus in Drosophila melanogaster . Molec. Gen. Genet. 182, 31–38 (1981). https://doi.org/10.1007/BF00422763

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  • DOI: https://doi.org/10.1007/BF00422763

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