Molecular and General Genetics MGG

, Volume 182, Issue 1, pp 19–24 | Cite as

The requirement for both DNA polymerase and 5′ to 3′ exonuclease activities of DNA polymerase I during Tn5 transposition

  • Chihiro Sasakawa
  • Yuko Uno
  • Masanosuke Yoshikawa
Article

Summary

By assaying transposition of Tn5 from λb221 cI857 rex::Tn5 (Berg 1977) in PolA-proficient and deficient cells, both the polymerase activity and 5′ to 3′ exonuclease acivity of DNA polymerase I have been shown to be required for transposition. This requirement could not be observed in three other systems in which the transposon donor replicon had existed in the PolA-proficient and deficient cells before the transposition event to be assayed occurred. By analogy to Tn3, this may indicate that the repressor encoded by Tn5 has already been expressed and hence become rate-limiting in the overall transposition process, even in PolA-deficient cells still possessing a residual activity. One polA mutant was found among more than 50 transposition-deficient (tnp) mutants isolated by the use of λb221 cI857 rex::Tn5.

Keywords

Residual Activity Polymerase Activity Deficient Cell Transposition Event polA Mutant 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Chihiro Sasakawa
    • 1
  • Yuko Uno
    • 1
  • Masanosuke Yoshikawa
    • 1
  1. 1.Institute of Medical ScienceUniversity of TokyoTokyoJapan

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