Cellular fatty acid composition of Oerskovia species, CDC Coryneform groups A-3, A-4, A-5, Corynebacterium aquaticum, Listeria denitrificans and Brevibacterium acetylicum
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Twenty four strains representing eight species of gram positive yellow-pigmented rods (Oerskovia turbata, Oerskovia xanthineolytica, CDC Coryneform groups A-3, A-4, A-5, Listeria denitrificans, Corynebacterium aquaticum and Brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. O. turbata, O. xanthineolytica, CDC groups A-3 and A-4, L. denitrificans and C. aquaticum were placed in the first group due to the presence of a higher percentage (29–47%) of 15:0a, than CDC group A-5 and B. acetylicum. The latter contained 2–6% of this fatty acid, and were placed in the second group.
All species in the two groups except C. aquaticum and CDC group A-4, were further separated from each other based on the qualitative and quantitative differences in their fatty acid compositions. In addition, the eight strains of CDC group A-5 revealed four different patterns and were further divided into four subgroups. This study supports the importance of the composition of cellular fatty acids in differentiating some closely related organisms.
Key wordscoryneform yellow-pigmented rods gas liquid chromatography fatty acid profiles
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- Berd D (1973) Laboratory identification of clinically important aerobic Actinomycetes. Appl. Microbiol. 25: 665–681Google Scholar
- Bidawid S, Chou S, Ng CW, Perry E & Kasatiya S (1989) Fatty acid profiles of Chlamydia using Capillary Gas Chromatography. Antonie Van Leeuwenhoek 55: 123–131Google Scholar
- Bousfield IJ, Smith GL, Dando TR & Hobbs G (1983) Numerical analysis of total fatty acid profiles in the identification of coryneform, nocardioform and other bacteria. J. Gen. Microbiol. 129: 375–394Google Scholar
- Coudron PE, Harris RC, Vaughan MG & Dalton HP (1985) Two similar but atypical strains of coryneform group A-4 isolated from patients with endophthalmitis. J. Clin. Microbiol. 22: 475–477Google Scholar
- Erikson D (1954) Factors promoting cell division in a ‘soft’ mycelial type of nocardia: Nocardia turbata n. sp. J. Gen. Microbiol. 11: 198–208Google Scholar
- Ewing WH & Davis BR (1970) Media and tests for differentiation of Enterobacteriacae. USPHS, Center for Disease Control. Atlanta, Ga.Google Scholar
- Hollis DG & Weaver RE (1981) Gram-positive Organisms: A Guide to Identification. USPHS, Center for Disease Control. Atlanta, Ga.Google Scholar
- Keddie RM & Bousfield IJ (1980) Cell wall composition in the classification and identification of coryneform bacteria. Soc. Appl. Bacteriol. Symp. Ser. 8: 167–188Google Scholar
- Lambert MA & Moss W (1983) Comparison of the effects of acid and base hydrolysis on hydroxy and cyclopropane fatty acid in bacteria. J. Clin. Microbiol. 18: 1370–1377Google Scholar
- Lechevalier MP (1972) Description of a new species, Oerskovia xanthineolytica, and emendation of Oerskovia Prauser et al. Int. J. Syst. Bacteriol. 22: 260–264Google Scholar
- Lechevalier MP (1977) Lipid in bacterial taxonomy — a taxonomist's view. CRC. Rev. Microbiol. 5: 109–210Google Scholar
- Na'Was TE, Hollis DG, Moss CW & Weaver RE (1987) Comparison of biochemical, morphologic and chemical characteristics of Centers for Disease Control fermentative coryneform groups 1, 2, and A-4. J. Clin. Microbiol. 25: 1354–1358Google Scholar
- Prauser H, Lechevalier MP & Lechevalier H (1970) Description of Oerskovia gen. n. to harbor Oerskov's motile nocardia. Appl. Microbiol. 19: 534Google Scholar
- Reller LB, Maddoux GL, Eckman MR & Pappas G (1975) Bacterial endocarditis caused by Oerskovia turbata. Ann. Int. Med. 83: 664–666Google Scholar
- Sottnek FO, Brown JM, Weaver RE & Carroll GF (1977) Recognition of Oerskovia species in the clinical laboratory: Characterization of 35 isolates. Int. J. Syst. Bacteriol. 27: 263–270Google Scholar