Advertisement

Archives of Microbiology

, Volume 128, Issue 3, pp 271–274 | Cite as

DNA-DNA hybridization of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides and Rhodopseudomonas sulfidophila strains

  • Jan A. M. de Bont
  • Annie Scholten
  • Theo A. Hansen
Article

Abstract

The genetic relatedness of 21 Rhodopseudomonas strains has been studied by means of DNA-DNA hybridization. All strains included in the study belonged to the subgroup of the genus Rhodopseudomonas which is characterized by a short-rod to coccus morphology, a vesicular intracytoplasmic membrane system and carotenoids of the spheroidene group. Mol percentages guanine + cytosine ranged from 64 to 73, most strains having values between 68 and 72. With few exceptions, the hybridization data obtained were in agreement with the subdivision in three (or possibly four) species on the basis of classical taxonomy. Strain SCJ, formerly considered to be a somewhat atypical R. capsulata strain, is most probably a R. sphaeroides strain and two out of seven strains that were received as R. sulfidophila did not fit in this species on the basis of the hybridization data. The results also showed that two undesignated strains that were previously thought to be related to R. capsulata (Hansen et al. 1975) cannot be assigned to this species and may be representatives of another species. The seven strains that required approximately 2.5% NaCl in the medium and that had been designated R. sulfidophila were found to synthesize far higher levels of bacteriochlorophyll during fully aerobic growth in the dark than the purple bacteria studied thus far.

Key words

DNA-DNA hybridization Genetic relatedness Rhodopseudomonas capsulata Rhodopseudomonas sphaeroides Rhodopseudomonas sulfidophila 

Abbreviations

GC

guanosine + cytosine

SSC

standard saline citrate buffer

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ambler RP, Daniel M, Hermoso J, Meyer TE, Bartsch RG, Kamen MD (1979) Cytochrome c 2 sequence variation among the recognised species of the purple nonsulfur photosynthetic bacteria. Nature 278:659–660Google Scholar
  2. de Bont JAM, Primrose SB, Collines MD, Jones D (1980) Chemical studies on some bacteria which utilize lower unsaturated hydrocarbons. J Gen Microbiol 117:97–102Google Scholar
  3. Britten RJ, Pavich R, Smith JA (1970) A new method for DNA purification. Carnegie Institute of Washington Year Book 68:400–402Google Scholar
  4. Cohen-Bazire G, Sistrom WR, Stanier RY (1957) Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68Google Scholar
  5. Commerford SL (1971) Iodination of nucleic acids in vitro. Biochemistry 10:1993–1998Google Scholar
  6. Crombach WHJ (1972) DNA base composition of soil arthrobacters and other coryneforms from cheese and sea fish. Antonie van Leeuwenhoek, J Microbiol Serol 38:105–120Google Scholar
  7. Dickerson RE (1980) Evolution and gene transfer in purple photosynthetic bacteria. Nature 283:210–212Google Scholar
  8. Eckersley K, Dow CS (1979) Rhodopseudomonas blastica, sp. n., a new species of the Rhodospirillaceae. In: Nichols JM (ed) Abstr. IIIrd Int Symp Photosynthetic Prokaryotes E12. Liverpool, University of LiverpoolGoogle Scholar
  9. Gibson J, Stackebrandt E, Zablen LB, Gupta R, Woese CR (1979) A phylogenetic analysis of the purple photosynthetic bacteria. Current Microbiol 3:59–64Google Scholar
  10. Hansen TA (1974) Sulfide als electrondonor voor Rhodospirillaceae. Ph D Thesis, University of GroningenGoogle Scholar
  11. Hansen TA, van Gemerden H (1972) Sulfide utilization by purple nonsulfur bacteria. Arch Mikrobiol 86:49–56Google Scholar
  12. Hansen TA, Sepers ABJ, van Gemerden H (1975) A new purple bacterium that oxidizes sulfide to extracellular sulfur and sulfate. Plant and Soil 43:17–29Google Scholar
  13. Hansen TA, Veldkamp H (1973) Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. Arch Mikrobiol 92:45–58Google Scholar
  14. Keppen OI, Nozhevnikova AN, Gorlenko VM (1976) Dark metabolism of Rhodopseudomonas sulfidophila. Microbiology 45:10–13Google Scholar
  15. Meyer TE (1979) Molecular definition of the bacterial species. In: Nichols JM (ed) Abstr. IIIrd Int Symp Photosynthetic Prokaryotes E12. Liverpool, University of LiverpoolGoogle Scholar
  16. Mandel M, Marmur J (1968) Use of ultraviolet absorbance-temperature profile for determing the guanine plus cytosine content of DNA. In: Grossman L, Moldave K (eds) Methods Enzymol 12B. Academic Press, New York London, pp 195–206Google Scholar
  17. van Niel CB (1944) The culture, general physiology, morphology and classification of the non-sulfur purple and brown bacteria. Bacteriol Rev 8:1–118Google Scholar
  18. Pfennig N (1974) Rhodopseudomonas globiformis, sp. n., a new species of the Rhodospirillaceae. Arch Microbiol 100:197–206Google Scholar
  19. Pfennig N (1977) Phototrophic green and purple bacteria: a comparative, systematic survey. Ann Rev Microbiol 31:275–290Google Scholar
  20. Pfennig N, Lippert KD (1966) Über das Vitamin-B 12-Bedürfnis phototropher Schwefelbakterien. Arch Mikrobiol 55:245–256Google Scholar
  21. Trüper HG, Pfennig N (1978) Taxonomy of the Rhodospirillales. In: Clayton RK, Sistrom WR (eds) The Photosynthetic Bacteria. Plenum, New York, pp 19–27Google Scholar
  22. Wall JD, Weaver PF, Gest H (1975) Gene transfer agents, bacteriophages, and bacteriocins of Rhodopseudomonas capsulata. Arch Microbiol 105:217–224Google Scholar
  23. Weaver PF, Wall JD, Gest H (1975) Characterization of Rhodopseudomonas capsulata. Arch Microbiol 105:207–216Google Scholar
  24. Weckesser J, Drews G, Mayer H (1979) Lipopolysaccharides of photosynthetic prokaryotes. Ann Rev Microbiol 33:215–239Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Jan A. M. de Bont
    • 1
  • Annie Scholten
    • 1
  • Theo A. Hansen
    • 1
  1. 1.Department of MicrobiologyAgricultural UniversityWageningenThe Netherlands

Personalised recommendations