Summary
Fatty acids of 18 strains representing 10 species of Thiobacillus were extracted from whole cells and examined as methyl esters by gas-liquid chromatography. Both visual and quantitative comparison of the resulting chromatograms for the presence and relative amounts of major peaks allowed rapid differentiation between such closely related species as Thiobacillus neapolitanus and T. thioparus and of eight other species. Except for a feature common to all thiobacilli tested, T. thiooxidans, T. neapolitanus and T. thioparus each possessed a characteristic fatty acid methyl ester profile that was exhibited by all the strains of that species. Hence, the thiobacilli could be divided into three distinct groups. It was possible to use the gas-liquid chromatographic patterns of the cellular fatty acids for rapid identification or grouping of these microorganisms since the fatty acid composition of the genus Thiobacillus thus appeared to be of taxonomic significance.
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Abbreviations
- GLC:
-
Gas-liquid chromatography
- FAME:
-
Fatty acid methyl ester(s)
References
Abel, K., DeSchmertzing, H., Peterson, J. I.: Classification of microorganisms by analysis of chemical composition. I. Feasibility of utilizing gas chromatography. J. Bact. 85, 1039–1044 (1963).
Agate, A. D., Korczynski, M. S., Lundgren, D. G.: Extracellular complex from the culture filtrate of Ferrobacillus ferrooxidans. Canad. J. Microbiol. 15, 259–264 (1969).
Agate, A. D., Vishniac, W.: Characterization of thiobacilli by gas-liquid chromatography. X th Internatl. Cong. Microbiol., Mexico City, Abstr., p. 43 (1970).
Henis, Y., Gould, J. R., Alexander, M.: Detection and identification of bacteria by gas chromatography. Appl. Microbiol. 14, 513–524 (1966).
Hutchinson, M., Johnstone, K. I., White, D.: The taxonomy of certain thiobacilli. J. gen. Microbiol. 41, 357–366 (1965).
Hutchinson, M., Johnstone, K. I., White, D.: Taxonomy of the acidophilic thiobacilli. J. gen. Microbiol. 44, 373–381 (1966).
Hutchinson, M., Johnstone, K. I., White, D.: Taxonomy of anaerobic thiobacilli. J. gen. Microbiol. 47, 17–23 (1967).
Jackson, J. M., Moriarty, D. J. W., Nicholas, D. J. D.: Deoxyribonucleic acid base composition and taxonomy of thiobacilli and some nitrifying bacteria. J. gen. Microbiol. 53, 53–60 (1968).
Korczynski, M. S., Agate, A. D., Lundgren, D. G.: Phospholipids from the chemoautotroph Ferrobacillus ferrooxidans. Biochem. biophys. Res. Commun. 29, 457–462 (1967).
Moss, C. W., Lewis, V. J.: Characterization of clostridia by gas chromatography. I. Differentiation of species by cellular fatty acids. Appl. Microbiol. 15, 390–397 (1967).
Short, S. A., White, D. C., Aleem, M. I. H.: Phospholipid metabolism in Ferrobacillus ferrooxidans. J. Bact. 99, 142–150 (1969).
Silberman, M. P., Lundgren, D. G.: Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J. Bact. 77, 642–647 (1959).
Vishniac, W., Santer, M.: The thiobacilli. Bact. Rev. 21, 195–213 (1957).
Youatt, J. B.: Studies on the metabolism of Thiobacillus thiocyanooxidans. J. gen. Microbiol. 11, 139–149 (1954).
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Agate, A.D., Vishniac, W. Characterization of Thiobacillus species by gas-liquid chromatography of cellular fatty acids. Archiv. Mikrobiol. 89, 257–267 (1973). https://doi.org/10.1007/BF00422206
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DOI: https://doi.org/10.1007/BF00422206