Summary
Increases in oxidative capacity of skeletal muscle which has undergone training is well documented. The concomitant shifts in mitochondrial size and cytochrome content have varied with different investigations. In this study the shifts in oxidative capacity, SDH activity, thiolase activity, was measured in two sizes of heart and skeletal muscle mitochondria of rats undergoing 4, 8, and 16 weeks of training. In addition cytochrome content was measured in both sizes of mitochondria of the skeletal muscle. The small mitochondria of skeletal muscle showed the significant gains in number during the first 8 weeks of training while the large mitochondria increased in number during the last 8 weeks of training. The oxygen consumption, SDH activity, thiolase activity and cytochrome content all showed varying peaks in activity and content in the different sizes of mitochondria over the 16 weeks of training. In evaluating training regimes' effects on skeletal muscle it appears important to take into consideration the shifts in size of mitochondria as well as oxidative capacity and enzymatic activity alterations. There were no alterations observed in mitochondrial size in cardiac tissue.
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References
Ariano MA, Armstrong RB, Edgerton VR (1973) Hindlimb muscle fiber population of five mammals. J Histochem Cytochem 21: 51–55
Barnard RJ, Edgerton VR, Peter JB (1970) Effect of exercise on skeletal muscle I. Biochemical and histochemical properties. J Appl Physiol 28: 762–766
Barnard RJ, Peter JB (1971) Effects of exercise on skeletal muscle III. Cytochrome changes. J Appl Physiol 31: 904–908
Bakeeva YS, Chentsov YS, Skulachev VP (1978) Mitochondrial framework (Reticulum mitochondriale) in rat diaphragm muscle. Biochem Biophys Acta 501: 349–369
Bostrom S, Fahlen M, Hjalmarson A, Johanson R (1974) Activities of rat muscle enzymes after acute exercise. Acta Physiol Scand 90: 544–554
Bonner WD (1975) Methods in enzymology, vol 1. In: Kaplan NO (ed). Academic Press, New York, p 722
Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. J Biol Chem 217: 383–393
Dohm GL, Huston RL, Askew EW, Weiser PC (1972) Effects of exercise on activity of heart and muscle mitochondria. Am J Physiol 223: 783–787
Dohm GL, Barakat H, Stephenson TP, Pennington SN, Tapscott EB (1975) Changes in muscle mitochondrial lipid composition resulting from training and exhaustive exercise. Life Sci 17: 1074–1080
Edington DW, Cosmas AC (1972) Effect of maturation and training on mitochondrial size distribution in rat hearts. J Appl Physiol 33: 715–718
Edington DW, McCafferty WB (1973) Mitochondrial size distribution analysis in the soleus muscle of trained and aged rats. Experientia 29: 692–693
Gollnick PD, King DW (1969) Effect of exercise and training on mitochondria of rat skeletal muscle. Am J Physiol 216: 1502–1509
Holloszy JO (1975) Adaptation of skeletal muscle to endurance exercise. Med Sci Sports 7: 155–164
Kiessling KH, Piehl K, Lundquist CG (1971) In: Pernow B, Saltin B (eds) Muscle metabolism during exercise. Plenum Press, New York, pp 97–101
Krieger DA, Tate CA, McMillin-Wood J, Booth FW (1980) Effect of exercise and disuse of two populations of rat skeletal muscle mitochondria. J Appl Physiol 48: 23–28
Lowry GH, Roseborough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Makinen MW, Lee CP (1968) Biochemical studies of skeletal muscle mitochondria. Arch Biochem Biophys 126: 75–82
Morgan RE, Cobb LA, Short FA, Ross R, Gunn DR (1971) In: Pernow B, Saltin B (eds) Muscle metabolism during exercise. Plenum Press, New York, pp 87–95
Morton DJ, Rowe RWD (1974) Ultrastructural modification of mitochondria of rat soleus muscle surgically induced to hypertrophy. Protoplasma 81: 335–348
Oscai LB, Mole PA, Brei B, Hollszy JO (1971) Cardiac growth and respiratory enzyme levels in male rats subjected to a running program. Am J Physiol 220: 1238–1241
Palmer JW, Tandler B, Hoppel CL (1977) Biochemical properties of subsarcolemmal and interfibrillar mitochondria isolated from rat cardiac muscle. J Biol Chem 252: 8731–8739
Swick RW, Stange JL, Nance SL, Thomson JF (1967) The heterogeneous distributions of mitochondrial enzymes in normal rat liver. Biochemistry 6: 737–744
Van Gelder BF, Slater EC (1962) The extinction coefficient of cytochrome c. Biochem Biophys Acta 58: 593–595
Vanneste WH (1966) Molecular proportion of fixed cytochrome components of the respiratory chain of Keilin-Hartree particles and beef heart mitochondria. Biochem Biophys Acta 113: 175–178
Ward CW, Fairbairn D (1970) Enzymes of B-oxidation and their function during development of Ascaris lumbricoides eggs. Dev Biol 22: 366–387
Welch MH, Clakson PM, Pearce TN, Edington DW (1975) Levels of RNA in response to exercise. Med Sci Sports 7: 64
Winder WW, Holloszy JO (1977) Response of mitochondria of different types of skeletal muscle thyrotoxicosis. Am J Phys 232: C180-C184
Williams JN (1964) A method for the simultaneous quantitative estimation of cytochromes a, b, c, and c1 in mitochondria. Arch Biochem Biophys 105: 537–543
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Farrar, R.P., Mayer, L.R., Starnes, J.W. et al. Selected biochemical parameters of two sizes of rat skeletal and heart muscle mitochondria at selected intervals of a 16-week endurance training program. Europ. J. Appl. Physiol. 46, 91–102 (1981). https://doi.org/10.1007/BF00422181
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DOI: https://doi.org/10.1007/BF00422181