Skip to main content
Log in

Metabolic types of muscle in the sheep: II. Lactate dehydrogenase activity and LDH isoenzyme distribution

  • Published:
European Journal of Applied Physiology and Occupational Physiology Aims and scope Submit manuscript

Summary

Lactate dehydrogenase activity and isoenzyme distribution were measured in fast-twitch red, slow-twitch red and fast-twitch white muscles in the sheep. Total enzyme activity is higher in fast-twitch muscles irrespective of their colour type. Isoenzymes M4 and H4 bear an inverse relationship, M4 being predominant in fast-twitch red and white muscles, and H4 in heart muscle and slow-twitch red muscles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

ATP:

Adenosine 5′ triphosphate

EDTA:

Ethylene diamine tetra acetic acid

NAD:

Nicotinamide adenine dinucleotide

References

  • Ansay M (1974) Individualité musculaire chez le bovin. Etude de l'équipement enzymatique de quelques muscles. Ann Biol Anim Biochem Biophys 14: 471–486

    Google Scholar 

  • Ashmore CR, Doerr L, Foster G, Carroll F (1971) Respiration of mitochondria isolated from dark cutting beef. J Anim Sci 33: 574–577

    Google Scholar 

  • Briand M, Talmant A, Briand Y, Monin G, Durand R (1981) Metabolic types of muscle in the sheep. I. Myosin ATPase, glycolytic, and mitochondrial enzyme activities. Eur J Appl Physiol 46: 347–358

    Google Scholar 

  • Cahn RD, Kaplan NO, Levine L, Zwilling E (1962) Nature and development of lactic dehydrogenases. Science 136: 962–969

    Google Scholar 

  • Dawson DM, Goodfriend TF, Kaplan NO (1964) Lactic dehydrogenases. Function of the two types. Science 43: 929–933

    Google Scholar 

  • Fritz PJ, Morrison WJ, White EL, Vesell ES (1970) Comparative study of methods for quantitation of lactate dehydrogenase isozymes. Anal Biochem 36: 443–453

    Google Scholar 

  • Kaplan NO (1964) Lactate dehydrogenase. Structure and function. Brookhaven Symp Biol 17: 131–153

    Google Scholar 

  • Karlsson J, Frith K, Sjödin B, Gollnick PD, Saltin B (1974) Distribution of LDH isozymes in human skeletal muscle. Scand J Clin Lab Invest 33: 307–312

    Google Scholar 

  • Peter JB, Sawaki S, Barnard RJ, Edgerton VR, Gillepsie CA (1971) Lactate dehydrogenase isoenzymes. Distribution in fast-twitch red, fast-twitch white, and slow-twitch intermediate fibers of guinea pig skeletal muscle. Arch Biochem Biophys 144: 304–307

    Google Scholar 

  • Peter JB, Barnard RJ, Edgerton VR, Gillepsie CA, Stempel E (1972) Metabolic profiles of three fiber types of skeletal muscles in guinea pigs and rabbits. Biochemistry 11: 2627–2633

    Google Scholar 

  • Tonomura Y (1972) Contraction of muscle models by ATP. Muscle proteins, Muscle contraction and cation transport. University Park Press, Baltimore, pp 238–258

    Google Scholar 

  • Van Wijhe M, Blanchaer MC, George-Stubbs ST (1964) The distribution of lactate dehydrogenase isoenzymes in human skeletal muscle fibers. J Histochem Cytochem 12: 608–614

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work was supported in part by DGRST. Comite technologique alimentaire et agricole. Contrat No. 78.700.30

Rights and permissions

Reprints and permissions

About this article

Cite this article

Briand, M., Talmant, A., Briand, Y. et al. Metabolic types of muscle in the sheep: II. Lactate dehydrogenase activity and LDH isoenzyme distribution. Europ. J. Appl. Physiol. 46, 359–365 (1981). https://doi.org/10.1007/BF00422123

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00422123

Key words

Navigation