Summary
Histochemical reactions and activities of mitochondrial enzymes in adipose tissue around the neck arteries and in pericardium were studied in men who had been outdoor workers in northern Finland. The purpose was to study the occurrence of brown fat in workers having been exposed to cool or cold ambient temperature. Indoor workers of the same age were used as controls.
Histochemically, no mitochondrial enzyme reactions were seen in the adipose tissues taken from the indoor workers, whereas some outdoor workers had some multilocular adipose tissue, mostly around the neck arteries. Biochemical parameters also showed increased enzyme activities of aerobic energy metabolism in the adipose tissue of these people.
The present results suggest that working in the cold can retain brown adipose tissue in “strategic” places in human adults.
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References
Aherne W, Hull D (1964) The site of heat production in the newborn infant. Proc R Soc Med 57: 1172–1173
Barka T, Anderson PJ (1965) Oxidative enzymes. In: Barka T, Anderson PJ (eds) Histochemistry. Hoeber Medical Division, New York Evanston London, pp 296–333
Brück K (1961) Temperature regulation in the newborn infant. Biol Neonat 3: 65–119
Brück K (1976) Cold adaptation in man. In: Janski L, Musacchia XJ (eds) Regulation of depressed metabolism and thermogenesis. Thomas, Springfield, pp 42–63
Bukowiecki L, Himms-Hagen J (1976) Alteration of mitochondrial protein metabolism in liver, brown adipose tissue and skeletal muscle during cold-acclimation. Biochem Biophys Acta 428: 591–599
Davis TRA (1961) Chamber cold acclimatization in man. J Appl Physiol 16: 1011–1015
Doi K, Ohno T, Kurahashi M, Kuroshima A (1979) Thermoregulatory nonshivering thermogenesis in man, with special reference to lipid metabolism. Jpn J Physiol 29: 359–372
Fawcett DW (1952) A comparison of the histological organization and cytochemical reactions of brown and white adipose tissue. J Morphol 90: 363–405
Foster DO, Frydman ML (1978) Nonshivering thermogenesis in the rat. II Measurements of blood flow with microspheres point to brown adipose tissue as the dominant site of the calorigenesis induced by noradrenaline. Can J Physiol Pharmacol 56: 110–122
Hassi J (1977) The brown adipose tissue in man. Structural and functional aspects in relation to age. Acta Univ Ouluensis [D] 21: 1–92
Hirvonen J, Weaver D, Williams DD (1973) Morphological and enzymehistochemical changes in the interscapular adipose tissue of adult guinea-pigs during prolonged exposure to cold. Experientia 26: 1566–1570
Huttunen P, Kinnula V (1979) Effects of catecholamine treatment as well as cold exposure on mitochondrial enzyme activities of the adipose tissue in a guinea-pig (Cavia porcellus). Comp Biochem Physiol 63: 13–16
Itoh S (1974) Physiology of cold-adapted man. Hokkaido University, School of Medicine, Sapporo
Joy RJT (1963) Responses of cold-acclimated men to infused norepinephrine. J Appl Physiol 18: 1209–1212
King TE (1967) Preparation of succinate dehydrogenase and reconstriction of succinate oxidase. Meth Enzym 10: 322–331
Kimura T, Hauter J, Singer TP (1967) Studies on succinate dehydrogenase. XIII. Reversible activation of the mammalian enzyme. J Biol Chem 242: 4987–4993
LeBlanc J (1975) Adaptation. In: LeBlanc J (ed) Man in the cold. Thomas, Springfield, pp 90–145
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-Phenol reagent. J Biol Chem 193: 265–275
Menschik Z (1953) Histochemical comparison of brown and white adipose tissue in guinea-pigs. Anat Rec 116: 439–455
Yonetani R, Ray GS (1965) Studies on cytochrome oxidase. VI. Kinetics of the aerobic oxidation of ferrocytochrome c by cytochrome oxidase. J Biol Chem 240: 3392–3398
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Huttunen, P., Hirvonen, J. & Kinnula, V. The occurrence of brown adipose tissue in outdoor workers. Europ. J. Appl. Physiol. 46, 339–345 (1981). https://doi.org/10.1007/BF00422121
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DOI: https://doi.org/10.1007/BF00422121