Summary
Ty4 is a novel transposable element in the yeast, Saccharomyces cerevisiae, which is present in only a few copies in the genome (Stucka et al. 1989). In strain C836 one of the three copies (Ty4-90) is contained in cosmid clone c90, where it resides on chromosome V. Analysis of this region reveals a “hot-spot” of transposition: in addition to Ty4-90, the locus contains a complete Ty3 element and seven singular delta, sigma and tau elements. Three tRNA genes (for His, Lys, and Ile) are located in this region, and these are closely associated with one or the other of the elements, a phenomenon commonly observed in yeast. A comparison of c90 with corresponding regions from other strains shows that the locus is highly polymorphic and that this polymorphism is explicitly associated with Ty transposition and recombination events.
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Abbreviations
- Ty :
-
Yeast transposable element
- ORF :
-
open reading frame
- LTRs :
-
long terminal repeats
References
Andreadis A, Hsu YP, Kohlhaw GB, Schimmel P (1982) Cell 31:319–325
Clark DJ, Bilanchone VW, Haywood LJ, Dildine SL, Sandmeyer SB (1988) J Biol Chem 262:1413–1423
DelRey F, Donahue TF, Fink GR (1982) J Biol Chem 258:8175–8182
Eibel H, Philippsen P (1984) Nature 286:414–418
Eigel A, Feldmann H (1982) EMBO J 1:1245–1255
Feldmann H (1988) In: Grunberg-Manago M (ed) Evolutionary tinkering in gene expression NATO Adv Study Inst, vol 69. Plenum Press, New York, pp 79–86
Felici F, Cesareni G (1987) Nucleic Acids Res 15:364
Fink GR, Boeke JD, Garfinkel DJ (1986) Trends Genet 2:118–124
Gafner J, de Robertis EM, Philippsen P (1983) EMBO J 2:583–591
Genbauffe FS, Chisholm GE, Cooper TG (1984a) Proc Natl Acad Sci USA 81:2965–2969
Genbauffe FS, Chisholm GE, Cooper TG (1984b) J Biol Chem 259:10518–10525
Hansen LJ, Chalker DL, Sandmeyer SB (1988) Mol Cell Biol 8:5245–5256
Hauber J, Nelböck P, Feldmann H (1985) Nucleic Acids Res 13:2745–2758
Hauber J, Stucka R, Krieg R, Feldmann H (1988) Nucleic Acids Res 16:10623–10634
Hohn B, Hinnen A (1980) In: Setlow JK, Hollaender A (eds) Genetic engineering, vol 2. Plenum Press, New York
Kingsman AJ, Kingsman SM (1988) Cell 53:333–335
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Marschalek R, Brechner T, Amon-Böhm E, Dingermann T (1989) Science 244:1493–1496
Nelböck P, Stucka R, Feldmann H (1985) Biol Chem Hoppe-Seyler 366:1041–1051
Picologlou S, Dicig ME, Kovarik P, Liebman SW (1988) Mol Gen Genet 211:272–281
Rothstein R, Helms C, Rosenberg N (1987) Mol Cell Biol 7:1198–1207
Sandmeyer SB, Bilanchone VW, Clark DJ, Morcos P, Carle GE, Brodeur GM (1988) Nucleic Acids Res 16:1499–1515
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sharp PM, Tuohy TMF, Mosurski KR (1986) Nucleic Acids Res 14:5125–5141
Smith CJ, Ley AN, D'Obrenan P, Mitra SK (1971) J Biol Chem 246:7817–7819
Stucka R, Hauber J, Feldmann H (1986) Curt Genet 11:193–200
Stucka R, Hauber J, Feldmann H (1987) Curr Genet 12:323–328
Stucka R, Lochmüller H, Feldmann H (1989) Nucleic Acids Res 17:4993–5001
Tschumper G, Carbon JA (1982) J Mol Biol 156:293–307
Warmington JR, Anwar R, Newlon CS, Waring RB, Davies RW, Inge KJ, Oliver SG (1986) Nucleic Acids Res 14:6679–6693
Warmington JR, Green RP, Newlon CS, Oliver SG (1987) Nucleic Acids Res 15:8963–8982
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Lochmüller, H., Stucka, R. & Feldmann, H. A hot-spot for transposition of various Ty elements on chromosome V in Saccharomyces cerevisiae . Curr Genet 16, 247–252 (1989). https://doi.org/10.1007/BF00422110
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DOI: https://doi.org/10.1007/BF00422110