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Morphogenetic and nutritional studies of Geotrichum lactis cells

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Summary

Geotrichum lactis was grown in culture media with different carbon and nitrogen sources. Biotin is needed as a growth factor. During incubation in shake flasks mycelium and arthrospores are produced. Under certain conditions, such as low pH, there is a new form that we have called “yeast-like form”. This thick-walled form develops from mature arthrospores. The arthrospore and the yeastlike cells have walls with different chemical and structural composition, as observed in the electron microscope, and in their resistance to some lytic extracts.

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References

  • Agar, H. D., Douglas, H. C.: Studies of budding and cell wall structure of yeast. J. Bact. 70, 427–434 (1955).

    Google Scholar 

  • Aguirre, M. J. R., Garcia Acha, I., Villanueva, J. R.: An enzyme from a Streptomyces sp. to prepare mould “protoplasts”. Experientia (Basel) 19, 82–83 (1963).

    Google Scholar 

  • Bartholomew, J. W., Levin R.: The structure of Saccharomyces carlsbergensis and Saccharomyces cerevisiae as determined by ultrathin sectioning methods and electron microscopy. J. gen Microbiol. 12, 473–477 (1955).

    Google Scholar 

  • Bartnicki-Garcia, S.: Mold-yeast dimorphism of Mucor. Bact. Rev. 27, 293–304 (1963).

    Google Scholar 

  • Butler, E. E.: Pathogenicity and taxonomy of Geotrichum candidum. Phytopathology 50, 665–672 (1960).

    Google Scholar 

  • Butler, E. E., Petersen, L. J.: Sexual reproduction in Geotrichum candidum. Science 169, 481–482 (1970).

    Google Scholar 

  • Butler, E. E., Petersen, L. J.: Endomyces geotrichum, a perfect state of Geotrichum candidum. Mycologia (N.Y.) 64, 365–374 (1972).

    Google Scholar 

  • Butler, E. E., Webster, R. K., Eckert, J. W.: Taxonomy, pathogenicity and physiological properties of the fungus causing sour rot of Citrus. Phytopathology 55, 1268 (1965).

    Google Scholar 

  • Carbonell, L. M.: Intrayeast hyphae in Paracoccidioides brasiliensis. J. Bact. 98, 1395–1396 (1969a).

    Google Scholar 

  • Carbonell, L. M.: Ultrastructure of dimorphic transformation in Paracoccidioides brasiliensis and brasiliensis. J. Bact. 100, 1076–1082 (1969b).

    Google Scholar 

  • Carbonell, L. M., Rodriguez, J.: Mycelial phase of Paracoccidioides brasiliensis and Blastomyces dermatitidis: an electron microscope study. J. Bact. 96, 533–543 (1968).

    Google Scholar 

  • Carmichael, J. W.: Geotrichum candidum. Mycologia (N.Y.) 49, 820–830 (1957).

    Google Scholar 

  • Cole, G. T., Kendrick, W. B.: Conidium ontogeny in Hyphomycetes. The arthrospores of Oidiodendron and Geotrichum and the endoarthrospores of Sporendonema. Canad. J. Bot. 47, 1773–1780 (1969).

    Google Scholar 

  • Feigl, F.: Spot test in organic analysis. Amsterdam-London-New York-Princeton: Elsevier Publ. Comp. 1956.

    Google Scholar 

  • García Acha, I., Aguirre, M. J. R., Lopez-Belmonte, F., Villanueva, J. R.: Use of lipase and strepzyme for the isolation of “protoplasts” from fungal mycelium. Nature (Lond.) 209, 95–96 (1966).

    Google Scholar 

  • Gascon, S., Ochoa, A. G., Villanueva, J. R.: Production of yeast and mold protoplasts by treatment with the strepzyme of Micromonospora AS. Canad. J. Microbiol. 11, 573–580 (1965).

    Google Scholar 

  • Houston, M. R., Meyer, K. H., Thomal, N., Wolf, F. T.: Dimorphism in Cladosporium werneckii. Sabouraudia 7, 195–198 (1969).

    Google Scholar 

  • Laborda, F.: Estudio sobre las paredes celulares de esporas de Fusarium culmorum. Tesis Doctoral. Facultad de Farmacia. Universidad de Santiago de Compostela (1971).

  • Lane, J. W., Garrison, R. G., Field, M. F.: Ultrastructural studies on the yeastlike and mycelial phase of Sporotrichum schenckii. J. Bact. 100, 1010–1019 (1969).

    Google Scholar 

  • Lodder, J.: Die Anaskosporogen Hefen, 1ste Hälfte, Verhandel. Akad. Wettenschappen amsterdam, Adfeel. Naturkunde. 2nd. Ser. 32, 1 (1934).

    Google Scholar 

  • Lodder, J. (Ed.): The yeast: A taxonomic study. Amsterdam: North-Holland Publ. Co. 1970

    Google Scholar 

  • Mardon, D., Balish, E., Phillips, A. W.: Control of dimorphism in a biochemical variant of Candida albicans. J. Bact. 100, 701–707 (1969).

    Google Scholar 

  • Park, D., Robinson, P. M.: Germination studies with Geotrichum candidum. Trans. Brit. mycol. Soc. 54, 83–92 (1970).

    Google Scholar 

  • Uruburu, F., Monreal, J.: Producción y ultraestructura del seudomicelio de Saccharomyces fragilis. Microbiol. Español. 20, 219–232 (1967).

    Google Scholar 

  • Van-Uden, N., do Carmo-Sousa, L.: Some physiological properties of Geotrichum candidum. Mycologia (N.Y.) 51, 595–598 (1959).

    Google Scholar 

  • Vitols, E., North, R. J., Linnane, A. W.: Studies on the oxidative metabolism of Saccharomyces cerevisiae. I. Observations on the fine structure of the yeast cell. J. biophys. biochem. Cytol. 9, 689–699 (1961).

    Google Scholar 

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Duran, A., Uruburu, F. & Villanueva, J.R. Morphogenetic and nutritional studies of Geotrichum lactis cells. Archiv. Mikrobiol. 88, 245–256 (1973). https://doi.org/10.1007/BF00421849

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