Summary
The phycoerythrins from Rhodomonas sp. strain 3-C and Cryptomonas ovata var. palustris were purified and partially characterized. The phycoerythrin from Rhodomonas had a single visible absorption maximum at 542 nm with a shoulder at approximately 562 nm and is, therefore, representative of cryptophyte type I phycoerythrin. The phycoerythrin from C. ovata var. palustris had a single absorption maximum at 566 nm and is, therefore, representative of cryptophyte type III phycoerythrin. Calibrated gel filtration chromatography showed that both of these phycoerythrins have a native molecular weight of 30 800 daltons. Calibrated sodium dodecyl sulfate gel electrophoresis demonstrated that both pigments were composed of two subunits with apparent molecular weights of 17 700 and 11 000 daltons. On polyacrylamide gel electrofocusing both these phycoerythrins had an isoionic point of 4.90.
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Brooks, C., Gantt, E. Comparison of phycoerythrins (542, 566 nm) from cryptophycean algae. Archiv. Mikrobiol. 88, 193–204 (1973). https://doi.org/10.1007/BF00421845
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DOI: https://doi.org/10.1007/BF00421845